Detail publikace

Causes and consequences of unique amorphous state of native granules of polyhydroxyalkanoates (PHAs) and its transitions into crystalline state

Originální název

Causes and consequences of unique amorphous state of native granules of polyhydroxyalkanoates (PHAs) and its transitions into crystalline state

Anglický název

Causes and consequences of unique amorphous state of native granules of polyhydroxyalkanoates (PHAs) and its transitions into crystalline state

Jazyk

en

Originální abstrakt

The role of PHAs is not only as carbon and energy source, but PHAs have also a protective effect for bacteria against various stress conditions (temperature, UV, osmotic stress). The unique properties of PHAs are closely connected with its mysterious amorphous structure in vivo. We assumed that PHAs amorphous state maintains due to presence of low molecular plasticizers (water, monomer molecules) despite of thermodynamical unfavorability. In our experiments, we used Cupriavidus necator H16 (CCM 3726) as a model PHA producing bacterial strain. We monitored mechanism of transition of amorphous PHAs into the crystalline form under various stress factors such as high temperature, cyclic freezing/thawing, high osmolality and low pH. For evaluation of morphological and structural changes accompanying crystallization of PHAs in–situ, we used various techniques such as ATR-FTIR, RAMAN, XRD, TEM.

Anglický abstrakt

The role of PHAs is not only as carbon and energy source, but PHAs have also a protective effect for bacteria against various stress conditions (temperature, UV, osmotic stress). The unique properties of PHAs are closely connected with its mysterious amorphous structure in vivo. We assumed that PHAs amorphous state maintains due to presence of low molecular plasticizers (water, monomer molecules) despite of thermodynamical unfavorability. In our experiments, we used Cupriavidus necator H16 (CCM 3726) as a model PHA producing bacterial strain. We monitored mechanism of transition of amorphous PHAs into the crystalline form under various stress factors such as high temperature, cyclic freezing/thawing, high osmolality and low pH. For evaluation of morphological and structural changes accompanying crystallization of PHAs in–situ, we used various techniques such as ATR-FTIR, RAMAN, XRD, TEM.

BibTex


@misc{BUT159303,
  author="Eva {Slaninová} and Petr {Sedláček} and Stanislav {Obruča}",
  title="Causes and consequences of unique amorphous state of native granules of polyhydroxyalkanoates (PHAs) and its transitions into crystalline state",
  annote="The role of PHAs is not only as carbon and energy source, but PHAs have also a protective effect for bacteria against various stress conditions (temperature, UV, osmotic stress). The unique properties of PHAs are closely connected with its mysterious amorphous structure in vivo. We assumed that PHAs amorphous state maintains due to presence of low molecular plasticizers (water, monomer molecules) despite of thermodynamical unfavorability. In our experiments, we used Cupriavidus necator H16 (CCM 3726) as a model PHA producing bacterial strain. We monitored  mechanism of transition of amorphous PHAs into the crystalline form under various stress factors such as high temperature, cyclic freezing/thawing, high osmolality and low pH. For evaluation of morphological and structural changes accompanying crystallization of PHAs in–situ, we used various techniques such as ATR-FTIR, RAMAN, XRD, TEM.
",
  booktitle="The Biomania Student scientific meeting - Eusynbios symposium 2019",
  chapter="159303",
  edition="1th",
  howpublished="print",
  year="2019",
  month="september",
  pages="116--117",
  type="abstract"
}