Causes and consequences of unique amorphous state of native granules of polyhydroxyalkanoates (PHAs) and its transitions into crystalline state

abstract

English

The role of PHAs is not only as carbon and energy source, but PHAs have also a protective effect for bacteria against various stress conditions (temperature, UV, osmotic stress). The unique properties of PHAs are closely connected with its mysterious amorphous structure in vivo. We assumed that PHAs amorphous state maintains due to presence of low molecular plasticizers (water, monomer molecules) despite of thermodynamical unfavorability. In our experiments, we used Cupriavidus necator H16 (CCM 3726) as a model PHA producing bacterial strain. We monitored mechanism of transition of amorphous PHAs into the crystalline form under various stress factors such as high temperature, cyclic freezing/thawing, high osmolality and low pH. For evaluation of morphological and structural changes accompanying crystallization of PHAs in–situ, we used various techniques such as ATR-FTIR, RAMAN, XRD, TEM.

Polyhydroxyalkanoates, stress conditions, crystallization, amorphous state, ATR-FTIR

SLANINOVÁ, E.; SEDLÁČEK, P.; OBRUČA, S.

30. 9. 2019

9788021093737

The Biomania Student scientific meeting - Eusynbios symposium 2019

1th

116

117

140