Czech

3

Not applicable.

Of all faculties

Practical aspects of various experimental techniques used in DNA research

It is necessary pass out lectures Bioanalytical methods.

Not applicable.

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Laboratory course is finished by identification of LAB using PCR

1. Safety of work in microbiological and biotechnological laboratory. Arrangement of PCR laboratory. 2. Cultivation of Lactobacillus acidophilus cells for isolation of chromosomal DNA. Cell lysis. 3. Removing of proteins using phenol extraction. Precipitation of DNA with ethanol. 4. Determination of DNA integrity using agarose gel electrophoresis. 5. Spectrophotometric estimation of concentration and purity of DNA. 6. Preparation of PCR mixture for genus specific amplification of Lactobacillus acidophilus using purified DNA as DNA matrix. Negative controls. 7. PCR amplification. Detection of PCR product using agarose gel electrophoresis. 8. Estimation of length of amplified fragment using external DNA standards. 9. Sensitivity of PCR. 10. Isolation of DNA from dairy product (yoghurt) . 11. Identification of Lactobacillus DNA in dairy product (yoghurt ) by PCR. Gel electophoresis of PCR amplicons. 12. -13. Test - identifikation of Lactobacillus cells in unknown sample using PCR.

Not applicable.

The laboratory course cover methodologies used in DNA analysis

Attendance in laboratory course is obligatory.

Not applicable.

Not applicable.

Španová A., Rittich B.: Analýza vybraných druhů baktérií mléčného kvašení pomocí metod molekulární biologie (CS)

Not applicable.

26 hours, compulsory