Czech

4

Not applicable.

Theoretical aspects of various isolation and separation techniques focused on microorganism cells

Without restriction

Not applicable.

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Oral examination

1. Stechiometry of microbial growth and material balances.
2. Systems with immobilized cells and enzymes.
3. Enzymatic catalysis in anhydrous and quasi anhydrous solvents.
4. Magnetic particles. Micromanipulation of particles.
5. Microflow in capillary tubes. Microdrops. Properties of fluids.
6. Mechanical, chemical and enzymatic cell disruption.
7. Precipitation and salting-out odf proteins. Filtration. Centrifugation.
8. Extraction - types of extractors and their design.
9. Extraction using aqueous two-phase systems.
10. Large scale chromatographic techniques.
11. Production of proteins using genetically modified microorganisms (GMO) and methods of their isolation.
12. Protein stability.
13. Validation of production quality.

Not applicable.

Lectures cover methods for biotechnoly product processing

Attendance at lectures is not obligatory but it is recommended. At seminars the calculations of model examples are given

Not applicable.

Not applicable.

Not applicable.

Not applicable.

13 hours, compulsory