Publication detail

Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection

VLČNOVSKÁ, M. ŠTOSSOVÁ, A. KUCHYNKA, M. DILLINGEROVÁ, V. POLANSKÁ, H. MASAŘÍK, M. HRSTKA, R. ADAM, V. KANICKÝ, V. VACULOVIČ, T. VACULOVIČOVÁ, M.

Original Title

Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection

Type

journal article in Web of Science

Language

English

Original Abstract

Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.

Keywords

protein p53; dot-blot; antibody

Authors

VLČNOVSKÁ, M.; ŠTOSSOVÁ, A.; KUCHYNKA, M.; DILLINGEROVÁ, V.; POLANSKÁ, H.; MASAŘÍK, M.; HRSTKA, R.; ADAM, V.; KANICKÝ, V.; VACULOVIČ, T.; VACULOVIČOVÁ, M.

Released

1. 2. 2021

Publisher

MDPI

ISBN

1420-3049

Periodical

MOLECULES

Year of study

26

Number

3

State

Swiss Confederation

Pages from

246

Pages to

259

Pages count

14

URL

https://www.mdpi.com/1420-3049/26/3/630

Full text in the Digital Library

http://hdl.handle.net/11012/196717

BibTex

@article{BUT171012,
  author="Marcela {Vlčnovská} and Aneta {Štossová} and Michaela {Kuchynka} and Veronika {Dillingerová} and Hana {Polanská} and Michal {Masařík} and Roman {Hrstka} and Vojtěch {Adam} and Viktor {Kanický} and Tomáš {Vaculovič} and Markéta {Vaculovičová}",
  title="Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection",
  journal="MOLECULES",
  year="2021",
  volume="26",
  number="3",
  pages="246--259",
  doi="10.3390/molecules26030630",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/26/3/630"
}