Detail publikace

Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection

VLČNOVSKÁ, M. ŠTOSSOVÁ, A. KUCHYNKA, M. DILLINGEROVÁ, V. POLANSKÁ, H. MASAŘÍK, M. HRSTKA, R. ADAM, V. KANICKÝ, V. VACULOVIČ, T. VACULOVIČOVÁ, M.

Originální název

Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.

Klíčová slova

protein p53; dot-blot; antibody

Autoři

VLČNOVSKÁ, M.; ŠTOSSOVÁ, A.; KUCHYNKA, M.; DILLINGEROVÁ, V.; POLANSKÁ, H.; MASAŘÍK, M.; HRSTKA, R.; ADAM, V.; KANICKÝ, V.; VACULOVIČ, T.; VACULOVIČOVÁ, M.

Vydáno

1. 2. 2021

Nakladatel

MDPI

ISSN

1420-3049

Periodikum

MOLECULES

Ročník

26

Číslo

3

Stát

Švýcarská konfederace

Strany od

246

Strany do

259

Strany počet

14

URL

https://www.mdpi.com/1420-3049/26/3/630

Plný text v Digitální knihovně

http://hdl.handle.net/11012/196717

BibTex

@article{BUT171012,
  author="Marcela {Vlčnovská} and Aneta {Štossová} and Michaela {Kuchynka} and Veronika {Dillingerová} and Hana {Polanská} and Michal {Masařík} and Roman {Hrstka} and Vojtěch {Adam} and Viktor {Kanický} and Tomáš {Vaculovič} and Markéta {Vaculovičová}",
  title="Comparison of metal nanoparticles (Au, Ag, Eu, Cd) used for immunoanalysis using LA-ICP-MS detection",
  journal="MOLECULES",
  year="2021",
  volume="26",
  number="3",
  pages="246--259",
  doi="10.3390/molecules26030630",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/26/3/630"
}