Publication detail

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

SVOBODA, O. FOHLEROVÁ, Z. BAIAZITOVA, L. ČMIEL, V. HUBÁLEK, J. PROVAZNÍK, I. PEKAŘÍK, V.

Original Title

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

English Title

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Type

conference paper

Language

en

Original Abstract

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

English abstract

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

Keywords

transfection, MATRA-A reagent, plasmid delivery

Released

06.05.2016

Publisher

Masarykova Univerzita - MUNI PRESS

Location

Brno, Czech Republic

ISBN

978-80-210-8267-0

Book

XVI. Workshop of Physical Chemists and Electrochemists

Edition

1

Pages from

1

Pages to

3

Pages count

3

BibTex


@inproceedings{BUT128268,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Baiazitova} and Vratislav {Čmiel} and Jaromír {Hubálek} and Ivo {Provazník} and Vladimír {Pekařík}",
  title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery",
  annote="The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.",
  address="Masarykova Univerzita - MUNI PRESS",
  booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
  chapter="128268",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Masarykova Univerzita - MUNI PRESS",
  year="2016",
  month="may",
  pages="1--3",
  publisher="Masarykova Univerzita - MUNI PRESS",
  type="conference paper"
}