Detail publikace

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Originální název

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Anglický název

MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery

Jazyk

en

Originální abstrakt

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

Anglický abstrakt

The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.

BibTex


@inproceedings{BUT128268,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Larisa {Baiazitova} and Vratislav {Čmiel} and Jaromír {Hubálek} and Ivo {Provazník} and Vladimír {Pekařík}",
  title="MATRA-A reagent/polyethyleneimine complex transfection for low toxic DNA plasmid delivery",
  annote="The non-toxic intracellular delivery of biopolymers is very hot topic in current biology. In this work, two different MATRA-A reagent/polyethyleneimine transfection protocols were used for intracellular delivery of ChR2 DNA vector. Cell morphology changes were studied 48 hours after transfection. Our transfection efficiency is lower than normally achieved, but the rate of cells with physiological properties remains is very high: 85.34%. Therefore, these transfection protocols are very applicable for electrophysiological experiments.",
  address="Masarykova Univerzita - MUNI PRESS",
  booktitle="XVI. Workshop of Physical Chemists and Electrochemists",
  chapter="128268",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Masarykova Univerzita - MUNI PRESS",
  year="2016",
  month="may",
  pages="1--3",
  publisher="Masarykova Univerzita - MUNI PRESS",
  type="conference paper"
}