Curriculum vitae

Education and academic qualification

• 2010, Ph.D., Faculty of Mechanical Engineering Brno University of Technology, Physical and Material Engineering (doctoral thesis: Microscopy of time variable biologic objects)
• 2006, Ing., Faculty of Mechanical Engineering Brno University of Technology, Fine Mechanics and Optics

Career overview

• 2008-01-01 - 2008-12-31

  Technical staffer, Institute of Physical Engineering, Faculty of Mechanical Engineering, BUT
  employment type 0.5

• 2009-01-01 - 2010-01-31

  Assistant, Institute of Physical Engineering, Faculty of Mechanical Engineering, BUT
  employment type 0.5

• 2010-02-01 - 2011-02-28

  Research assistant, Institute of Physical Engineering, Faculty of Mechanical Engineering, BUT
  employment type 1.0

• 2011-07-01 - 2015-11-10

  Postdoctoral Fellow, Neurovascular Imaging, UCSD, California
  employment type 1.0

• 2015-11-11 - ...

  Research assistant, Institute of Physical Engineering, Faculty of Mechanical Engineering, BUT
  employment type 0.1

• 2015-11-11 - ...

  Research Engineer, CEITEC, BUT
  employment type 0.2

Pedagogic activities

2006-2010 Tutorials in Mechanics, Electricity and Magnetism, Optics, Thermodynamics, Vibration and Waves

2009-2010 Lecture Basics od fluorescence microscopy

2010-2012 Supervision of Diploma and Bachelor theses (Aneta Jebackova, Lukas Hladik, Jan Bartonicek)

12/2013 Dynamic imaging in neuroscience (IBRO), 2 week-internation college: lecture and experimental demonstrations

Scientific activities

• Kivilcim Kilic, Hana Uhlirova, Peifang Tian, Mohammad Abbas Yaseen, Jonghwan Lee, Sergei A. Vinogradov,  David A. Boas, Sava Sakadzic, Anna Devor. Tools for high resolution in vivo imaging of cellular and molecular mechanisms in cortical spreading depression and spreading depolarization. In: Neurobiological Basis of Migraine. Wiley. In print.

• Uhlirova H, Kilic K, Tian P, Sakadzic S, Gagnon L, Thunemann M, Desjardins M, Saisan PA, Nizar K, Yaseen MA, Hagler DJ Jr, Vandenberghe M, Djurovic S, Andreassen OA, Silva GA, Masliah E, Kleinfeld D, Vinogradov S, Buxton RB, Einevoll GT, Boas DA, Dale AM, Devor A. The roadmap for estimation of cell-type-specific neuronal activity from non-invasive measurements. Philos Trans R Soc Lond B Biol Sci. 2016 Oct 5;371(1705).

• H. Uhlirova , K. Kilic , P. Tian , M. Thunemann , M. Desjardins , P. Saisan , S. Sakadzic , T. Ness , C. Mateo, Q. Cheng , K. Weldy , F. Razoux , M. Vanderberghe , J. Cremonesi, K. Nizar , V. Sridhar , T. Steed , M. Abashin , S. Fainman , E.r Masliah , S. Djurovic , O. Andreassen , G. Silva , D. Boas , D. Kleinfeld , R. Buxton , G. T. Einevoll , A. Dale. Cell type specificity of neurovascular coupling in cerebral cortex. eLIFE 5 (2016), p. e14315.

• K. Nizar, H. Uhlirova, P. Tian, P. A. Saisan, Q. Cheng, L. Reznichenko, K. L. Weldy, T. C. Steed, V. B. Sridhar, C. L. MacDonald, J. Cui, S. L. Gratiy, S. Sakadzic, D. A. Boas, T. I. Beka, G. T. Einevoll, J. Chen, E. Masliah, A. M. Dale, G. A. Silva, A. Devor. J Neurosci 33, pp. 8411-22 (2013), ISSN: 0270-6474.

• H. Janeckova, P. Vesely, R. Chmelik, Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy. Anticancer Research 29, pp. 2339-2346 (2009), ISSN: 0250-7005.

• P. Kolman, H. Janeckova, R. Chmelik, Digital holographic microscopy at the IPE, BUT. Fine mechanics and optics (Czech) 7-8, 206-208 (2009), ISSN: 0447-6441.

• H. Janeckova, P. Kolman, P. Vesely, R. Chmelik, Digital holographic microscope with low spatial and temporal coherence of illumination. In: Optical and digital image processing, Proc of SPIE, 7000, c. 700002E, pp. 1 - 8, 2008 (ISSN: 0277-786X, ISBN: 9780819471987).

Academic internships abroad

• 02/2005–04/2005 Internship in the Laboratory of Hard Tissue Research, Centre of Oral Growth and Development (prof. Alan Boyde).

• 05/2005–07/2005 Internship in the Laboratory of Light Microscopy, Cancer Research UK (Ing. Daniel Zicha, Ph.D.).

Keywords

microscopy, two-photon micorsopy, fluorescence microscopy, brain imaging, calcium imaging, fluorescence lifetime, neurovascular coupling, NADH, electrophysiology, uncaging, holographic microscopy, biological applications of microscopy

Projects

•  2011 - 2015 Neuronal, glial and BOLD fMRI Signals: From BOLD to 2-photon microscopy (R01 NS057198-01, PI: Anna Devor, PhD)

•  2011 - 2015 Spatiotemporal Brain Imaging: Microscopic and Systems Level (R01EB000790-06, PI: Anders Dale, PhD)

• 2006 Image reconstruction of threedimensional objects by the means of holographic confocal microscopy (PI: doc. RNDr. Radim Chmelík,Ph.D.).

• 2007 Microscopy of time variable biologic objects, project of the Faculty of Mechanical Engineering (PI: Ing. Hana Janečková) – the project was evaluated as the winner in the application category.

• since 2008 Function hybrid nanosystems of semiconductors and metals with organic materials (FUNS), research project within the programme “Nanotechnology for society” (PI: RNDr. Bohuslav Rezek, Ph.D.).

• 2008–2010 The utilization of digital holographic microscope for research of live cell dynamics and completion of the development of a novel multifunction microscope (PI: doc. RNDr. Radim Chmelík, Ph.D.).