Detail publikace

Měření depozice vláken v modelu lidských plic metodou fázově kontrastní mikroskopie s automatickou analýzou obrazu

LÍZAL, F. ELCNER, J. BĚLKA, M. JEDELSKÝ, J. HOPKE, P. ŠTARHA, P. DRUCKMÜLLEROVÁ, H. JÍCHA, M.

Originální název

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

Český název

Měření depozice vláken v modelu lidských plic metodou fázově kontrastní mikroskopie s automatickou analýzou obrazu

Anglický název

Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis

Typ

článek v časopise

Jazyk

en

Originální abstrakt

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Český abstrakt

Depozice vláknových částic v lidském dýchacím ústrojí představuje známé zdravotní riziko. In-vitro měření byla provedena za pomoci monodispersních skleněných vláken v realistickém modelu lidských plic do sedmé generace větvení pro určení vlivu vláken a jejich velikosti na režim dýchání a depozici v plicích. Usazená vlákna byla dále vymyta ze segmentů modelu a zachycena na filtrech. Fázová kontrastní microskopie s kamerou s vysokým rozlišením byla použita při nasnímání vláken usazených na filtrech. Dále byl vyvinut software pro automatizaci analýzy nasnímaných obrazů pro lepší výpočet depozice skleněných vláken. Tato metoda se jeví jako užitečná a hodnotná pro stanovování depozice vláken a tuhých částic.

Anglický abstrakt

Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.

Klíčová slova

Lidské plíce, Depozice, Vlákna, Analýza obrazu

Rok RIV

2013

Vydáno

20.12.2013

Nakladatel

Association for Engineering Mechanics

Místo

Brno

Strany od

187

Strany do

194

Strany počet

8

BibTex


@article{BUT96300,
  author="František {Lízal} and Jakub {Elcner} and Miloslav {Bělka} and Jan {Jedelský} and Philip K. {Hopke} and Pavel {Štarha} and Hana {Druckmüllerová} and Miroslav {Jícha}",
  title="Measurement of Fiber Deposition in a Human Lung Model by Phase Contrast Microscopy with Automated Image Analysis",
  annote="Deposition of fibers in human lungs is known as a health hazard. In-vitro measurements were performed with monodisperse glass fibers in a realistic model of human lungs up to seventh generation of branching to estimate the effect of fiber size and breathing pattern on fiber deposition. Deposited fibers were rinsed from the model segments and gathered on nitrocellulose filters. Phase-contrast microscopy with high resolution camera was used to capture images of filters with fibers. New software was developed for an automated image analysis and local deposition characteristics were calculated afterwards. The whole method proved to be a useful and valuable tool for the evaluation of fiber and particle deposition.",
  address="Association for Engineering Mechanics",
  chapter="96300",
  institution="Association for Engineering Mechanics",
  number="3/4",
  volume="20",
  year="2013",
  month="december",
  pages="187--194",
  publisher="Association for Engineering Mechanics",
  type="journal article"
}