Detail publikace

Recording of monophasic action potentials during ischemia - reperfusion by optical method

NOVÁKOVÁ, M. BARDOŇOVÁ, J. PROVAZNÍK, I.

Originální název

Recording of monophasic action potentials during ischemia - reperfusion by optical method

Český název

Recording of monophasic action potentials during ischemia - reperfusion by optical method

Typ

článek v časopise - ostatní, Jost

Jazyk

cs

Originální abstrakt

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

Český abstrakt

The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.

Klíčová slova

Isolated rabbit heart, Ischemia, Reperfusion, Monophasic action potential, Electrogram

Rok RIV

2007

Vydáno

23.12.2007

Místo

Brno

Strany od

1

Strany do

13

Strany počet

13

BibTex


@article{BUT45329,
  author="Marie {Nováková} and Jana {Kolářová} and Ivo {Provazník}",
  title="Recording of monophasic action potentials during ischemia - reperfusion by optical method",
  annote="The present paper deals with new possibility how to study ischemia and reperfusion in rabbit isolated heart perfused according to Langendorff. Electrogram was recorded by touch-less method and coronary flow measured throughout the experiment. Moreover, monophasic action potentials (MAPs) were obtained by optical probe using voltage-sensitive dye di-4-ANEPPS. Each experiment consisted from five successive periods: stabilization (control), staining with the dye, washout of the dye, flow ischemia, reperfusion. During ischemic period, typical changes of electrogram were observed and they were accompanied by corresponding changes of MAPs. In conclusion, MAPs recorded by optical method can be used as a tool for studying ischemia - reperfusion changes in isolated rabbit hearts. However, since the effects of the dye to tissue itself are under intensive investigation and not fully elucidated yet, it is necessary to be somewhat cautious about the results obtained in this way.",
  chapter="45329",
  journal="Scripta medica",
  number="6",
  volume="2007",
  year="2007",
  month="december",
  pages="1--13",
  type="journal article - other"
}