Detail publikace

Cell-free DNA in plasma as an essential immune system regulator

Korabecna, M. Zinkova, A. Brynychova, I. Chylikova, B. Prikryl, P. Sedova, L. Neuzil, P. Seda, O.

Originální název

Cell-free DNA in plasma as an essential immune system regulator

Anglický název

Cell-free DNA in plasma as an essential immune system regulator

Jazyk

en

Originální abstrakt

The cell-free DNA (cfDNA) is always present in plasma, and it is biomarker of growing interest in prenatal diagnostics as well as in oncology and transplantology for therapy efficiency monitoring. But does this cfDNA have a physiological role? Here we show that cfDNA presence and clearance in plasma of healthy individuals plays an indispensable role in immune system regulation. We exposed THP1 cells to healthy individuals’ plasma with (NP) and without (TP) cfDNA. In cells treated with NP, we found elevated expression of genes whose products maintain immune system homeostasis. Exposure of cells to TP triggered an innate immune response (IIR), documented particularly by elevated expression of pro-inflammatory interleukin 8. The results of mass spectrometry showed a higher abundance of proteins associated with IIR activation due to the regulation of complement cascade in cells cultivated with TP. These expression profiles provide evidence that the presence of cfDNA and its clearance in plasma of healthy individuals regulate fundamental mechanisms of the inflammation process and tissue homeostasis. The detailed understanding how neutrophil extracellular traps and their naturally occurring degradation products affect the performance of immune system is of crucial interest for future medical applications.

Anglický abstrakt

The cell-free DNA (cfDNA) is always present in plasma, and it is biomarker of growing interest in prenatal diagnostics as well as in oncology and transplantology for therapy efficiency monitoring. But does this cfDNA have a physiological role? Here we show that cfDNA presence and clearance in plasma of healthy individuals plays an indispensable role in immune system regulation. We exposed THP1 cells to healthy individuals’ plasma with (NP) and without (TP) cfDNA. In cells treated with NP, we found elevated expression of genes whose products maintain immune system homeostasis. Exposure of cells to TP triggered an innate immune response (IIR), documented particularly by elevated expression of pro-inflammatory interleukin 8. The results of mass spectrometry showed a higher abundance of proteins associated with IIR activation due to the regulation of complement cascade in cells cultivated with TP. These expression profiles provide evidence that the presence of cfDNA and its clearance in plasma of healthy individuals regulate fundamental mechanisms of the inflammation process and tissue homeostasis. The detailed understanding how neutrophil extracellular traps and their naturally occurring degradation products affect the performance of immune system is of crucial interest for future medical applications.

Dokumenty

BibTex


@article{BUT165719,
  author="Pavel {Neužil}",
  title="Cell-free DNA in plasma as an essential immune system regulator",
  annote="The cell-free DNA (cfDNA) is always present in plasma, and it is biomarker of growing interest in
prenatal diagnostics as well as in oncology and transplantology for therapy efficiency monitoring. But
does this cfDNA have a physiological role? Here we show that cfDNA presence and clearance in plasma
of healthy individuals plays an indispensable role in immune system regulation. We exposed THP1 cells
to healthy individuals’ plasma with (NP) and without (TP) cfDNA. In cells treated with NP, we found
elevated expression of genes whose products maintain immune system homeostasis. Exposure of
cells to TP triggered an innate immune response (IIR), documented particularly by elevated expression
of pro-inflammatory interleukin 8. The results of mass spectrometry showed a higher abundance of
proteins associated with IIR activation due to the regulation of complement cascade in cells cultivated
with TP. These expression profiles provide evidence that the presence of cfDNA and its clearance in
plasma of healthy individuals regulate fundamental mechanisms of the inflammation process and
tissue homeostasis. The detailed understanding how neutrophil extracellular traps and their naturally
occurring degradation products affect the performance of immune system is of crucial interest for
future medical applications.",
  address="Springer",
  chapter="165719",
  doi="10.1038/s41598-020-74288-2",
  howpublished="online",
  institution="Springer",
  number="1",
  volume="10",
  year="2020",
  month="december",
  pages="17478-1--17478-10",
  publisher="Springer",
  type="journal article in Web of Science"
}