Detail publikace

Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques

Originální název

Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques

Anglický název

Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques

Jazyk

en

Originální abstrakt

Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. This statement is based on our observation that PHA accumulating cells of Cupriavidus necator H16 survived exposure to UV irradiation challenge much better than its PHA non-accumulating mutant. Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere, nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm.

Anglický abstrakt

Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. This statement is based on our observation that PHA accumulating cells of Cupriavidus necator H16 survived exposure to UV irradiation challenge much better than its PHA non-accumulating mutant. Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere, nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm.

BibTex


@misc{BUT159300,
  author="Eva {Slaninová} and Petr {Sedláček} and Filip {Mravec} and Ondrej {Hesko} and Lucie {Müllerová} and Stanislav {Obruča}",
  title="Protective effect of PHA granules in bacterial cells against UV-irradiation investigated by various spectral techniques",
  annote="Polyhydroxyalkanoates (PHA) are polyesters accumulated in form of intracellular granules by numerous bacteria primarily as storage compounds, nevertheless, we investigated that PHA granules are not used only as carbon and energy storage but bacteria can use this compound also as UV-protectant. This statement is based on our observation that PHA accumulating cells of Cupriavidus necator H16 survived exposure to UV irradiation challenge much better than its PHA non-accumulating mutant. Mechanisms of protective effect of PHA granules were determined by different spectroscopic approaches such as turbidity measurement, absorption spectrophotometry with integration sphere, nephelometry and continuous fluorescence microphotolysis. Generally, protective mechanisms of PHA granules is predominantly based on very effective scattering of UV radiation with wavelength of about 250 – 290 nm.",
  address="C.A.R.M.E.V. e.V.",
  chapter="159300",
  howpublished="print",
  institution="C.A.R.M.E.V. e.V.",
  year="2019",
  month="september",
  pages="88--88",
  publisher="C.A.R.M.E.V. e.V.",
  type="abstract"
}