Detail publikace

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Originální název

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Anglický název

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Jazyk

en

Originální abstrakt

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

Anglický abstrakt

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

BibTex


@inproceedings{BUT138959,
  author="Larisa {Baiazitova} and Vratislav {Čmiel} and Josef {Skopalík} and Ondřej {Svoboda} and Ivo {Provazník}",
  title="Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells",
  annote="Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.",
  booktitle="2017 25th European Signal Processing Conference (EUSIPCO)",
  chapter="138959",
  howpublished="online",
  year="2017",
  month="august",
  pages="469--473",
  type="conference paper"
}