Detail publikace

Investigation of interaction between magnetic silica particles andlambda phage DNA fragment

Originální název

Investigation of interaction between magnetic silica particles andlambda phage DNA fragment

Anglický název

Investigation of interaction between magnetic silica particles andlambda phage DNA fragment

Jazyk

en

Originální abstrakt

Nucleic acids belong to the most important molecules and therefore the understanding of theirproperties, function and behavior is crucial. Even though a range of analytical and biochemicalmethods have been developed for this purpose, one common step is essential for all of them –isolation of the nucleic acid from the from complex sample matrix. The use of magnetic parti-cles for the separation of nucleic acids has many advantages over other isolation methods. In thisstudy, an isolation procedure for extraction of DNA was optimized. Each step of the isolation pro-cess including washing, immobilization and elution was optimized and therefore the efficiency wasincreased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to thepreviously described method. Quantification of the particular parameter influence was performed bysquare-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimizedmethod with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureusand Escherichia coli.

Anglický abstrakt

Nucleic acids belong to the most important molecules and therefore the understanding of theirproperties, function and behavior is crucial. Even though a range of analytical and biochemicalmethods have been developed for this purpose, one common step is essential for all of them –isolation of the nucleic acid from the from complex sample matrix. The use of magnetic parti-cles for the separation of nucleic acids has many advantages over other isolation methods. In thisstudy, an isolation procedure for extraction of DNA was optimized. Each step of the isolation pro-cess including washing, immobilization and elution was optimized and therefore the efficiency wasincreased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to thepreviously described method. Quantification of the particular parameter influence was performed bysquare-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimizedmethod with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureusand Escherichia coli.

BibTex


@article{BUT102368,
  author="Kristýna {Šmerková} and Simona {Dostálová} and Markéta {Vaculovičová} and Jindřich {Kynický} and Libuše {Trnková} and Miroslav {Králík} and Vojtěch {Adam} and Jaromír {Hubálek} and Ivo {Provazník} and René {Kizek}",
  title="Investigation of interaction between magnetic silica particles andlambda phage DNA fragment",
  annote="Nucleic acids belong to the most important molecules and therefore the understanding of theirproperties, function and behavior is crucial. Even though a range of analytical and biochemicalmethods have been developed for this purpose, one common step is essential for all of them –isolation of the nucleic acid from the from complex sample matrix. The use of magnetic parti-cles for the separation of nucleic acids has many advantages over other isolation methods. In thisstudy, an isolation procedure for extraction of DNA was optimized. Each step of the isolation pro-cess including washing, immobilization and elution was optimized and therefore the efficiency wasincreased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to thepreviously described method. Quantification of the particular parameter influence was performed bysquare-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimizedmethod with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureusand Escherichia coli.",
  chapter="102368",
  doi="10.1016/j.jpba.2013.07.039",
  number="1",
  volume="86",
  year="2013",
  month="december",
  pages="65--72",
  type="journal article in Web of Science"
}