Detail publikace

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

KUNISHIMA, N. TAKEDA, Y. HIROSE, R. KALASOVÁ, D. ŠALPLACHTA, J., OMOTE, K.

Originální název

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.

Klíčová slova

3D rendering; Germination; Pansy seeds; Tomography; X-ray microscope

Autoři

KUNISHIMA, N.; TAKEDA, Y.; HIROSE, R.; KALASOVÁ, D.; ŠALPLACHTA, J., OMOTE, K.

Vydáno

1. 2. 2020

Nakladatel

BioMed Central

ISSN

1746-4811

Periodikum

PLANT METHODS

Ročník

16

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

10

Strany počet

10

URL

Plný text v Digitální knihovně

BibTex

@article{BUT161932,
  author="Naoki {Kunishima} and Yoshihiro {Takeda} and Raita {Hirose} and Dominika {Kalasová} and Jakub {Šalplachta} and Kazuhiko {Omote}",
  title="Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography",
  journal="PLANT METHODS",
  year="2020",
  volume="16",
  number="1",
  pages="1--10",
  doi="10.1186/s13007-020-0557-y",
  issn="1746-4811",
  url="http://link.springer.com/article/10.1186/s13007-020-0557-y"
}