Publication detail

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

KUNISHIMA, N. TAKEDA, Y. HIROSE, R. KALASOVÁ, D. ŠALPLACHTA, J., OMOTE, K.

Original Title

Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

Type

journal article in Web of Science

Language

English

Original Abstract

The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorbing biological samples. Here we report the observation of unstained pansy seeds using the laboratory-based X-ray phase-contrast CT.

Keywords

3D rendering; Germination; Pansy seeds; Tomography; X-ray microscope

Authors

KUNISHIMA, N.; TAKEDA, Y.; HIROSE, R.; KALASOVÁ, D.; ŠALPLACHTA, J., OMOTE, K.

Released

1. 2. 2020

Publisher

BioMed Central

ISBN

1746-4811

Periodical

PLANT METHODS

Year of study

16

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

10

Pages count

10

URL

http://link.springer.com/article/10.1186/s13007-020-0557-y

Full text in the Digital Library

http://hdl.handle.net/11012/193426

BibTex

@article{BUT161932,
  author="Naoki {Kunishima} and Yoshihiro {Takeda} and Raita {Hirose} and Dominika {Kalasová} and Jakub {Šalplachta} and Kazuhiko {Omote}",
  title="Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography",
  journal="PLANT METHODS",
  year="2020",
  volume="16",
  number="1",
  pages="1--10",
  doi="10.1186/s13007-020-0557-y",
  issn="1746-4811",
  url="http://link.springer.com/article/10.1186/s13007-020-0557-y"
}