Doctoral Thesis

Study of Scattering and Polarization of Light in Biological Tissue

Final Thesis 1.71 MB Summary of Thesis 610.9 kB

Author of thesis: Hamed Mohamed Abubaker, Ph.D.

Acad. year: 2012/2013

Supervisor: prof. RNDr. Pavel Tománek, CSc.

Reviewer's: prof. RNDr. Miroslav Hrabovský, DrSc.,


Tissue optics becomes a rapidly expanding field of great interest and a precise knowledge of optical properties of biological tissues is essential for biomedical investigation and food quality control.
If the sample of tissue is illuminated, the multiple scattering occurs. In the case of the postmortem tissue (meat) the cell dimensions are larger than the wavelength. Mie scattering of transmitted or reflected light arises and produces various polarization states.
Polarization properties of light scattered from a scattering medium have been studied with experiments and modeling. Two modified experiments were performed: scattering of polarized light passing twice the sample (forward and backward) and only transmitted light.  The measurements of scattered light display depolarization and rotation of polarized light, which depend both on orientation of the muscle fibers and ageing process of meat.  Theoretical description of turbid biological tissue and computing of radiative transfer equation by modified Polarized Light Monte Carlo (PLMC) method has also been executed.
It is shown that the degree of polarization is sensitive to the optical properties of the turbid medium. The results demonstrate that polarized light scattered from a scattering medium is sensitive to the state of input polarization and the optical properties and thickness of the tissue during the ageing. The correlations of polarization changes and freshness of meat, as well as dynamic behavior of the polarization in ageing meat are shown.


biological tissue, light, scattering, polarization, Stokes vector, Mueller matrix, Monte Carlo, ageing, computing, measurement

Date of defence


Result of the defence

Defended (thesis was successfully defended)


Language of thesis




Study branch

Physical Electronics and Nanotechnology (PP-FEN)

Composition of Committee

prof. Ing. Lubomír Grmela, CSc. (předseda)
doc. RNDr. Milada Bartlová, Ph.D. (člen)
Prof. RNDr. Miroslav Hrabovský, DrSc. - oponent (člen)
Ing. Ondřej Číp. Ph.D. - oponent (člen)
Doc. Dr. Ing. Josef Lazar (člen)
Prof. RNDr. Dr. Jiří Luňáček (člen)

Supervisor’s report
prof. RNDr. Pavel Tománek, CSc.

viz příloha v pdf
File inserted by supervisor Size
posudek-Abubaker.pdf 58.8 kB

viz posudek ve formátu pdf
File inserted by the reviewer Size
Hrabovský_posudek_Abubaker.pdf 1.74 MB