Course detail

Technology in Cellular Engineering

FEKT-MPA-TBIAcad. year: 2020/2021

Not applicable.

Learning outcomes of the course unit

Not applicable.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Alberts B., Johnson A., Walter P.: Molecular Biology of the Cell. (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Not applicable.

Language of instruction

English

Work placements

Not applicable.

Course curriculum

1. Cultivation and preparation of cells and tissues, automation of processes in the cell laboratory.
2. Mechanical properties and methods of their measurement in tumor cells. Holographic microscopy and its application.
3. Spectroscopic methods in cellular engineering, micro-volume spectrophotometry.
4. Special microscopic imaging techniques in cellular engineering. Recording techniques for microscopy.
5. Electron microscopy in cellular engineering, correlation microscopy, preparation of biological samples for electron microscopy.
6. Methods of measurement of electrical activity of excitable tissue using microelectrodes and microelectrode systems.
7. Cell counting and sorting technologies, automated laboratory analyzers for tissue engineering.
8. Methods and technologies for cell migration analysis.
9. Technologies in regenerative and cancer medicine.
10. Technologies and methods used in optogenetics.
11. Microfluidic systems and their use in cellular engineering.
12. Methods of measurement of processes of excitation-contraction binding in muscle cells and tissues.

Aims

Not applicable.

Classification of course in study plans

  • Programme MPA-BIO Master's, 2. year of study, summer semester, 5 credits, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

Exercise in computer lab

13 hours, compulsory

Teacher / Lecturer

Laboratory exercise

13 hours, compulsory

Teacher / Lecturer