Course detail

Analytical Chemistry II

FCH-BC_ANC2Acad. year: 2019/2020

This course aims to introductuion students to the basic methods and techniques of instrumental analytical chemistry. Teaching is structured in the following thematic areas: Physical aspects of analytical methods. Analytical signal and its qulitative and quantitative evaluation. Principles of selected instrumental methods-spectrometric and electroanalytical methods, their qualitative and quantitative aspects and practical examples. Potentiometry. Voltametry and polarography (classical, pulse, stripping). Titrations with polarizable electrodes. Electrogavimetry, coulometry. Conductometry, dielectrimetry. Optical methods, their classification, properties of electromagnetic radiation. Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. Electronic absorption spectra, instrumentation. UV-VIS molecular spectrophotometry. Luinescence methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence. Absorption and emission atomic spectrometry. Principles and importance of chromatographic and electromigration methods. Introduction to analytical chemometry. Methods of quantitative evaluation.

Learning outcomes of the course unit

By completion of the course the student will gain the following knowledge and skills:
1. They will know in depth the principles of simple instrumental analytical methods and possibilities of their application.
2. They will be able to select the optimal method for solving of specific analytical problems of analytical practice.
3. They will adopt the basic principles of chemometric treatment of analytical results obtained using simple instrumental-analytical methods.

Prerequisites

Knowledge from the course Analytical chemistry I, knowledge from physics (electricity, magnetism), knowledge from physical chemistry

Co-requisites

Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Sommer L.: Základy analytické chemie II. VUTIUM, Brno 1998. (CS)
Volka K.: Analytická chemie II. VŠCHT Praha, 1997. (CS)
Christian G.D.: Analytical chemistry. Wiley, 2003. (CS)
Vláčil F. a kol.: Příklady z chemické a instrumentální analýzy. SNTL Praha, 1978; Informatorium Praha, 1991. (CS)
Kotouček M., Skopalová J.: Příklady z analytické chemie. Vydavatelství Univerzity Palackého Olomouc, 1997. (CS)
E-learningový kurs na https://moodle.vutbr.cz/course/view.php?id=185312 (CS)
Douglas A. Skoog et.al.: Analytická chemie, 2019 (CS)
K. Záruba a spol.: Analytická chemie 1. a 2. díl, 2016 (CS)

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Course credit is awarded for 100% attendance at seminars (any absence must be properly apologized). Credit may be obtained for successful completing of three partial written tests during the semester (ie getting at least 6 points out of 10 possible). In case of failure in one partial test (or more) course credit could be awarded for the successful completing of the final test (ie, obtaining at least 60 points out of 100).
Condition for passing the exam is to obtain credit from the exercise of Analytical Chemistry II.
Examination consists of a written and oral part, evaluate the level of knowledge of theory and practice of analytical chemistry and creative approach to solving analytical problems.

Language of instruction

Czech

Work placements

Not applicable.

Course curriculum

1. Introduction to instrumental analysis. Position and importance of intrumental analytical techniques, their historical development, definition of basic concepts.

Topic I: Electroanalytical Methods
2. Potentiometry
3. Voltammetry and polarography (classical, differential pulse, dissolution, adsorption). Potentiometric dissolution analysis, titration with polarizable electrodes. Electrogravimetry, Coulometry, Conductometry.

Topic II: Spectrometry
4. Classification of spectral methods, basic physical principles, properties of electromagnetic radiation. Non-spectral methods: Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. UV-VIS spectrophotometry - principle, properties, device design, application.
5. Luminescent methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence and their analytical use.
6. Mass spectrometry.
7. Fundamentals of optical atomic spectrometry.

Topic III: Separation Methods
Basic theory of chromatographic separation process. Planar Chromatography, Column Chromatography.
9. Liquid chromatography
10. Gas chromatography.
11. Separation in the electric field. Planar techniques (gel electrophoresis), capillary techniques (capillary zone electrophoresis, isotachophoresis, capillary isoelectric focusation).
12. Tandem techniques.

Topic IV: Evaluation of results
13. Basics of analytical chemometry. Procedures for evaluating analytical results and calibration functions.

Aims

The aim of the course is to provide students with an overview of selected instrumental analytical methods from the field of electrochemistry, spectrophotometry and separation techniques and to familiarize them with their application fields and their limitations.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is recommended, but not checked. Attendance at seminars is compulsory and is checked.
The students' work is controlled by three partial written tests during the semester in computational exercises.
For students in combined form of study are organized consultations on the topics for full-time students.
Consultations are also provided to full-time students on request.

Classification of course in study plans

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_SCH , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_SCH , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_CHTOZP , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_CHTOZP , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_CHMN , 2. year of study, summer semester, 6 credits, compulsory
    branch BPCO_CHM , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_CHM , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHCHTE Bachelor's, 2. year of study, summer semester, 6 credits, compulsory
  • Programme BKCP_CHCHTE Bachelor's, 2. year of study, summer semester, 6 credits, compulsory
  • Programme BPCP_CHTOZP Bachelor's, 2. year of study, summer semester, 6 credits, compulsory
  • Programme BKCP_CHTOŽP Bachelor's, 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHTP Bachelor's

    branch BPCO_BT , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHTP Bachelor's

    branch BKCO_BT , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHTPO Bachelor's

    specialization PCH , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHTPO Bachelor's

    specialization PCH , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHTP Bachelor's

    branch BKCO_PCH , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHTP Bachelor's

    branch BPCO_CHP , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHTPO Bachelor's

    specialization BT , 2. year of study, summer semester, 6 credits, compulsory
    specialization CHPL , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BKCP_CHTPO Bachelor's

    specialization BT , 2. year of study, summer semester, 6 credits, compulsory
    specialization CHPL , 2. year of study, summer semester, 6 credits, compulsory

  • Programme BPCP_CHTM Bachelor's, 2. year of study, summer semester, 6 credits, compulsory
  • Programme BKCP_CHTM Bachelor's, 2. year of study, summer semester, 6 credits, compulsory
  • Programme AKREDITACE revize Bachelor's, 2. year of study, summer semester, 6 credits, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

eLearning