FEKT-FMOLAcad. year: 2018/2019
Course is conceived as an introduction into molecular biology. It gives the explanation of processes on molecular level in relation to the basic functions of living systems (procaryotic as well as eucaryotic organisms and viruses). On the whole, the general molecular processes occuring in living systems are emphasized with respect to the specificities characteristic of the main groups of living systems (bacteria, archea, plants, animals and viruses).
Learning outcomes of the course unit
The student will be able to:
- describe principles of processes in living organisms on molecular level,
- explain gene expression,
- describe principle of cell signalling,
- discus changes of genetic information.
The student should be able to explain fundamental principles of genetics, should know basic terms and laws of molecular biology. Generally, knowledge on the level of Bachelor study is required.
Recommended optional programme components
Recommended or required reading
Rosypal S. a kol. : Úvod do molekulární biologie. I.-IVdíl. Brno
1999-2002 (třetí vydání). 2006 - I. díl (čtvrté vydání) (CS)
Rosypal S. a kol.: Terminologie molekulární biologie, Brno 2001. (CS)
Šmarda J. a kol.. Metody molekulární biologie, Brno, 2005. (CS)
Alberts et al.: Molecular biology of the cell. Garland Publ. 2004. (EN)
Alberts a kol: Základy buněčné biologie, Espero, 2000, 2005. (CS)
Relichová, J. Genetika populací. Masarykova univerzita v Brně, 1997, ISBN 80-210-1542 (CS)
Relichová, J. Praktická cvičení z genetiky. Masarykova univerzita v Brně, 1997, ISBN 80- 210-1484-9 (96 stran) Relichová, J. (1997): Praktická cvičení z genetiky. Masarykova univerzita v Brně, 1997, ISBN 80- 210-1484-9 (CS)
Planned learning activities and teaching methods
Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.
Assesment methods and criteria linked to learning outcomes
up to 40 points from computer exercises (2 tests, 1 homework, and activity in labs)
up to 60 points from finel written exam
The exam is oriented to verification of orientation in advanced terms of molecular biology.
Language of instruction
1. Introduction to the study of molecular biology. Cell, the chemical composition of cells, chemical energy, protein: structure and function, structure and function of nucleic acids.
2. Genetic information, genetic code, gene definitions, types of gene structure and information content of genomes.
3. Replication of DNA (the participating enzymes and mechanisms).
4. Transcription in prokaryotes and eukaryotes, posttranscriptional RNA editing.
5. Translation, transfer RNA, ribosomes, cotranslational and posttranslational processes.
6. Regulation of gene expression in prokaryotes, structure and properties of the promoter, the positive and negative regulation of operon, attenuation.
7. Regulation of gene expression in eukaryotes, transcription factors, transcriptional. Cell division, cell cycle control.
8. Molecular basis for the development of immunite molecules.
9. Cell signaling (signal molecules, receptors, signaling pathways). The structure of the membranes, transfer of substances through the membrane, the communication between cells.
10. Changes in genetic information, mutation, general and site-specific recombination, repair of damaged DNA mutant.
11. Mobile elements (prokaryotic and eukaryotic transposons, retrotransposons). Tissues.
12. Introduction of genetic engineering, production of transgenic organisms, and their use, in vitro mutagenesis, gene therapy.
13. Basics of using biochemical methods in molecular biology.
The aim of the course is to provide knowledge of differences of eukaryots, prokaryots and viruses in molecular biology.
Specification of controlled education, way of implementation and compensation for absences
Computer exercises are obligatory. Excused absence can be substituted.