Basic Principles of Modern Biotechnologies
FCH-BCO_ZMAcad. year: 2018/2019
Students will be introduced to biotechnological processes used for production of selected microbial, mammalian, hybridome and plant substances. Further, principles of fermentation procedures, separation of cells from media, dissintegration processes, separation techniques and purification of biomolecules will be discussed. As a part of this course basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC will be introduced. Methods of identification of recombinant DNA will be explained. Gene expression in microbial (bacteria, yeast) and animal expression systems will be described including expression markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection) will be introduced too.
Learning outcomes of the course unit
Principles and theoretical basis of modern biotechnologies, mainly gene technologies and some kinds of their applications. Novel strategies in gene engineering.
Biology, Biochemistry 1, Molecular biology
Recommended optional programme components
Recommended or required reading
Alberts a kol.: Základy buněčné biologie. Espero Publishing, 2001. (CS)
Rosypal S.: Úvod do molekulární biologie I-IV. Brno, 2002. (CS)
Hartl D.L:, Jones E.W.: Genetics: Analysis of Genes and Genomes. Jones and Bartlett Publishers 2001 (EN)
e-learning FCH (CS)
Planned learning activities and teaching methods
The course uses teaching methods in form of Lecture - 1 teaching hour per week, Seminar - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Writing test and oral examination. Elaboration and presentation of seminary project.
Language of instruction
1. Basic terms of molecular genetics; structure and function of information macromolecules.
2. Methods of isolation and analysis of biomolecules
3. Transformation vectors, restriction endonucleases and other enzymes used to analysis and processing of nucleic acids.
5. Methods of transformation, selection criteria of recombninants.
6. Clonning of DNA, polymerase chain reaction, use of PCR to clinical and food diagnostics.
7. Molecular analysis of genome - sequencing. Gene libraries.
8. Mutagenesis - random, site-specific.
9. Production, isolation and analysis of recombinant proteins.
10. Preparation of transgenic organisms, optimization of transgene expression.
11. Genetically modified microorganisms and their applications.
12. Transformation of higher organisms - plant, animals.
Selection of biological system for production of nucleic acids, proteins and for gene engineering. Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).
Specification of controlled education, way of implementation and compensation for absences
Participation at lectures is recommended. Presence at seminars is obligatory.