FCH-BCO_GETAcad. year: 2018/2019
Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).
Learning outcomes of the course unit
Fundamental principles and theoretical knowledge of gene technologies and their biotechnological applications. New strategies in genetic engineering.
Examination - Biochemistry I. Basic knowledge about structure and function of biomolecules.
Recommended optional programme components
Recommended or required reading
Rosypal S.: Úvod do molekulární biologie. Brno, 2002 (CS)
Alberts a kol. : Základy buněčné biologie. Espero Publishong 2001. (CS)
Hartl D.L:, Jones E.W.: Genetics: Analysis of Genes and Genomes. Jones and Bartlett Publishers 2001. (CS)
/e-learning FCH (CS)
Planned learning activities and teaching methods
The course uses teaching methods in form of Lecture - 2 teaching hours per week, Seminar - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Examination - written and oral part. Elaboration and prezentation of seminar project.
Language of instruction
1. Basic terms of molecular genetics; structure and function of information macromolecules
2. Overview of methods for isolation and analysis of nucleic acids
3. Transformation vectors, restriction endonucleases and other enzymes used in recombinant DNA technology
4. Hybridizationof nucleic acids.
5. Methods of transformation, criteria for recombinant cell selection.
6. Clonning of DNA, polymerase chain reaction and its applications in clinical and food diagnostics.
7. Molecular analysis of genome - sequenation. Gene libraries.
9. Production, isolation and analysis of recombinant proteins.
10. Construction of transgenic organisms, optimalization of transgene expression.
11. Genetically modified microoorganisms and their applications.
12. Transformation of higher organisms - plants, animals.
13. Overview of applications of genetic engineering in food processing and biotechnology, gene therapy.
This course is focused on introduction of basic methods and techniques used in gene technology and recombinant DNA technology: isolation, characterization and analysis of nucleic acids, DNA clonning, polymerase chain reaction (PCR), gene mapping, methods of transformation, types of transformation vectors, identification of recombinant DNA, foreign gene expression in prokaryotic and eukaryotic expression systems, identification and purification of expressed recombinant proteins.
Specification of controlled education, way of implementation and compensation for absences
Attendance at lectures is not compulsory, but is recommended. Attendance at seminars is compulsory and controlled by lecturer.
Classification of course in study plans
- Programme BPCP_CHTP Bachelor's
branch BPCO_BT , 3. year of study, summer semester, 4 credits, compulsory
- Programme BKCP_CHTP Bachelor's
branch BKCO_BT , 3. year of study, summer semester, 4 credits, compulsory
- Programme CKCP_CZV lifelong learning
branch CKCO_CZV , 1. year of study, summer semester, 4 credits, compulsory