Methods for Analysis of Biological Systems
FCH-BCA_MABSAcad. year: 2017/2018
The aim of the course is to present methods which are used in chemistry, biochemistry and biotechnology in order to separate, characterize, identify and quantify various biomolecules. The presented methods includes centrifugation, dialysis and ultrafiltration. Furthermore, the course will be focused also on chromatography (gel filtration, ion-exchange chromatography, HIC, affinity chromatography) and its utilization for isolation and purification of proteins and other biomolecules. Moreover, attention will be paid also to electrophoretic techniques used for characterization of proteins (PAGE, PAGE-SDS, Tricine-PAGE, native PAGE, 2D electrophoresis etc.) and DNA/RNA (horizontal electrophoresis, DGGE, TGGE etc.). The aim of the course will be also to explain principles of other methods for characterization of molecular mass of biopolymers such as SEC/MALS, ultracentrifugation, MALDI-TOF, DLS and others. In order to understand structure and function of biomolecules, the students will be familiarized with UV-VIS spectrometry, fluorescence spectroscopy, mass spectrometry, microfluidic techniques, flow cytometry and antibody-based assays . The course will also deals with application of optical, fluorescence and electron microscopy to study complex biological systems.
Learning outcomes of the course unit
The students attending the course will gain knowledge as well as practice with selected methods and techniques utilized for analysis of complex biological systems.
basic knowledge on biology, biochemistry and molecular biotechnology, understanding of basic properties of biomolecules and methods employed in analytical chemistry
Recommended optional programme components
Recommended or required reading
Králová B. : Bioanalytické metody. Vysoká škola chemicko-technologická v Praze, Praha 2001. (CS)
Lodish H., Berk A., Matsudaira P., Kaiser C.A., Krieger M., Scott M.P., Zipursky L., Darnell J. : Molecular Cell Biology. W. H. Freeman and Company, New York 2001. (CS)
Štulík K. : Analytické separační metody. Nakladatelství Karolinum, Praha 2004. (CS)
Voet D., Voetová J.G. : Biochemie. Victoria Publishing, Praha 1995. (CS)
Planned learning activities and teaching methods
The course uses teaching methods in form of Lecture - 2 teaching hours per week, Seminar - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Examination - written and oral part. Elaboration and prezentation of seminar project.
Language of instruction
1. Characterization of basic biomolecules (proteins, DNA, RNA, lipids etc.), structure, function and properties
2. Characterization, quantification and purification of proteins, electrophoretic techniques, activity assays, determination of molecular weight, basic methods utilized in proteomics, protein sequencing
3. Characterization, quantification, purification and amplification of nucleic acids, electrophoretic techniques
4. Optical, fluorescence and electron microscopy in analysis of biological systems
5. Flow cytometry
6. ELISA other antibody-based assays
7. Modern fluorescence methods in analysis of biological systems
8. Determination of gene expression
The aim of the course is to familiarize students with instrumental methods utilized for separation and analysis of biological systems, special attention will be paid to methods used in nanototechnology and nanobiotechnology.
Specification of controlled education, way of implementation and compensation for absences