prof. RNDr. Ivana Márová, CSc.

Institute of Food Science and Biotechnology (ÚCHPBT)

After passing of the course Biochemistry I is student mastered of overview about cell composition, structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, basic knowledge about structure of biomemrbanes and types of membrane transport are introduced and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, overview about methods of isolation and characterization of biopolymers will be obtained and some applications including enzyme engineering and genetic engineering will be discussed.

Knowledge of organic chemistry, basic knowledge of physical chemistry.

Not applicable.

Not applicable.

Alberts B. Bray G. a kol: Základy buněčné biologie. Espero Publishing, 2. vyd., Praha 2005 Alberts, B., Bray, D. a kol. : Základy buněčné biologie. 2.vyd., Espero Publishing, Liberec 2005. (CS)
Vodrážka Z. : Biochemie. Academia, Praha 1996. (CS)
Voet D., Voet J.G. : Biochemie. Victoria Publishing, Praha 1990. (CS)
Nelson D.L., Cox M.M. : Lehninger Biochemistry. W.H. Freemann and Co., New York 2007. (CS)
Karlson P. : Zaklady biochemie. Academia, Praha 1981. (CS)
Vodrážka Z. : Biochemie. Academia, Praha 1996. (CS)

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.

a) exercises are necessary; 6 partial tests should be written before oral examination (min. 65% of each)
b) oral examination - combination od 3 questions from the whole course; overview of chemical and metabolical parts and their logical interconnection

Czech

Not applicable.

Organization and composition of living cells and cellular compartments.
Amino acids - composition and structure, peptides, proteins.
Methods of isolation, purification and separation of proteins.
Functional roles of proteins.
Carbohydrates - monosaccharides, polysaccharides, glycoproteins.
Lipids - structure and classification. Triglycerides, phospholipids. Isoprenoids.
Biomembranes. Membrane transport.
Enzymology, enzyme catalysis. Substrate specificity. Cofactors. Regulation of enzyme activity.
Enzyme kinetics, Michaelis-Menten equation. Inhibition of enzymes.
Metabolic pathways. termodynamic relationships and energy-rich components.
Nucleic acids - structure and function. DNA, RNA.
Applide biochemistry - modern biotechnology, genetic engineering.

The aim of the course Biochemistry I is to introduce cell composition and to characterize structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, structure of biomemrbanes and types of membrane transport are explained and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, methods of isolation and characterization of biopolymers are introduced and some applications including enzyme engineering and genetic engineering are discussed.

Exercises are obligatory, lectures recommended.

• Programme BPCP_CHCHT Bachelor's

  branch BPCO_SCH , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BKCP_CHCHT Bachelor's

  branch BKCO_SCH , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BPCP_CHCHT Bachelor's

  branch BPCO_CHTOZP , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BKCP_CHCHT Bachelor's

  branch BKCO_CHTOZP , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BPCP_CHCHT Bachelor's

  branch BPCO_CHMN , 2. year of study, summer semester, 4 credits, compulsory
  branch BPCO_CHM , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BKCP_CHCHT Bachelor's

  branch BKCO_CHM , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BPCP_CHTP Bachelor's

  branch BPCO_BT , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BKCP_CHTP Bachelor's

  branch BKCO_BT , 2. year of study, summer semester, 4 credits, compulsory
  branch BKCO_PCH , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme BPCP_CHTP Bachelor's

  branch BPCO_CHP , 2. year of study, summer semester, 4 credits, compulsory
  

• Programme CKCP_CZV lifelong learning

  branch CKCO_CZV , 1. year of study, summer semester, 4 credits, compulsory
  

13 hours, compulsory

prof. RNDr. Ivana Márová, CSc.
Ing. Petra Matoušková, Ph.D.
doc. Ing. Stanislav Obruča, Ph.D.