Course detail

Biochemistry I

FCH-BCA_BCH1Acad. year: 2017/2018

Organization and composition of living cells and cellular compartments. Amino acids - composition and structure, peptides, proteins. Methods of isolation and separation of proteins. Functional roles of proteins. Nucleic acids. Carbohydrates. Lipids. Biomembranes. Metabolic pathways. termodynamic relationships and energy-rich components. Enzymology, enzyme catalysis. Enzyme kinetics, inhibition of enzymes. Regulation of enzyme activity.

Learning outcomes of the course unit

After passing of the course Biochemistry I is student mastered of overview about cell composition, structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, basic knowledge about structure of biomemrbanes and types of membrane transport are introduced and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, overview about methods of isolation and characterization of biopolymers will be obtained and some applications including enzyme engineering and genetic engineering will be discussed.

Prerequisites

Knowledge of organic chemistry, basic knowledge of physical chemistry.

Co-requisites

Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

a) exercises are necessary; 6 partial tests should be written before oral examination (min. 65% of each)
b) oral examination - combination od 3 questions from the whole course; overview of chemical and metabolical parts and their logical interconnection

Language of instruction

Czech

Work placements

Not applicable.

Course curriculum

Organization and composition of living cells and cellular compartments.
Amino acids - composition and structure, peptides, proteins.
Methods of isolation, purification and separation of proteins.
Functional roles of proteins.
Carbohydrates - monosaccharides, polysaccharides, glycoproteins.
Lipids - structure and classification. Triglycerides, phospholipids. Isoprenoids.
Biomembranes. Membrane transport.
Enzymology, enzyme catalysis. Substrate specificity. Cofactors. Regulation of enzyme activity.
Enzyme kinetics, Michaelis-Menten equation. Inhibition of enzymes.
Metabolic pathways. termodynamic relationships and energy-rich components.
Nucleic acids - structure and function. DNA, RNA.
Applide biochemistry - modern biotechnology, genetic engineering.

Aims

The aim of the course Biochemistry I is to introduce cell composition and to characterize structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, structure of biomemrbanes and types of membrane transport are explained and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, methods of isolation and characterization of biopolymers are introduced and some applications including enzyme engineering and genetic engineering are discussed.

Specification of controlled education, way of implementation and compensation for absences

Exercises are obligatory, lectures recommended.

Zařazení předmětu ve studijních plánech

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_SCH , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_SCH , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_CHTOZP , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_CHTOZP , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BPCP_CHCHT Bachelor's

    branch BPCO_CHMN , 2. year of study, summer semester, 4 credits, compulsory
    branch BPCO_CHM , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BKCP_CHCHT Bachelor's

    branch BKCO_CHM , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BPCP_CHTP Bachelor's

    branch BPCO_BT , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BKCP_CHTP Bachelor's

    branch BKCO_BT , 2. year of study, summer semester, 4 credits, compulsory
    branch BKCO_PCH , 2. year of study, summer semester, 4 credits, compulsory

  • Programme BPCP_CHTP Bachelor's

    branch BPCO_CHP , 2. year of study, summer semester, 4 credits, compulsory

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1. year of study, summer semester, 4 credits, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

eLearning