Course detail

Biosensors

FEKT-ABSNAcad. year: 2012/2013

The subject deals with a wide spectra of chemical sensors and biosensors used in medicine and related branches, with the principles of magnitude conversion, modern construction and applications of sensors in disease diagnostics, further in analyses and monitoring of toxic and biologically interesting matters. Another offer is the choice of physical magnitude sensors as flowmeters and pressure and temperature sensors.

Language of instruction

Czech

Number of ECTS credits

5

Mode of study

Not applicable.

Guarantor

prof. Ing. Jaromír Hubálek, Ph.D.

Department

Department of Microelectronics (UMEL)

Learning outcomes of the course unit

Basic overview of types and principles of sensors used in medicine, acquisition of theoretical knowledge and practical experience in the field of sensors.

Prerequisites

Knowledge at secondary school level and of completed subjects in the study area

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

Requirements for successful completion of the subject are specified in guarantor‘ s regulation updated for every academic year.

Course curriculum

The subject goes through five basic conversion principles of chemical and biochemical magnitudes of sensors used in medicine. In addition to the basic principles there are modern microsensor constructions to be shown, as well as the influence of miniaturization on the parameters of microsensors and their applications in use.

1. Introduction, basic chemical sensors and biosensors, definition, principle of measurements
2. Electrochemical sensors and microsensors, impedimetric sensors
3. Potentiometric sensors, pH sensors, ion-selective sensors, ISFET sensors, LAPS biosensors
4. Polarographic, voltammetric and amperometric sensors
5. Gravimetric chemo- and biosensors and microsensors
6. Temperature and calorimetric biosensors
7. Optical chemo- and biosensors and microsensors
8. Fluorescent chemo- and biosensors and microsensors, chemiluminescence, bioluminiscence
9. Special biosensors, enzymatic biosensors, transducers for bioafinite sensors
10. Imunosensors, electrochemical biosensors for DNA
11. Physical sensors - temperature, flow, energy, pressure, humidity, magnetic sensors, Hall`s Phenomenon

Work placements

Not applicable.

Aims

The aim of the subject is to give an overview of the basic physical, chemical and biochemical principles and characteristics of the basic sensor types used in medicine, as well as for the construction of analyzers and other medical devices.

Specification of controlled education, way of implementation and compensation for absences

Extent and forms are specified by guarantor’s regulation updated for every academic year.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Skládal, P.: Biosensory, Masarykova univerzita, Brno 2002 (CS)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme BTBIO-A Bachelor's

    branch A-BTB , 3. year of study, winter semester, compulsory

  • Programme EEKR-CZV lifelong learning

    branch ET-CZV , 1. year of study, winter semester, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

doc. Ing. Jana Drbohlavová, Ph.D.
doc. Mgr. Markéta Vaculovičová, Ph.D.

Syllabus

1. Introduction, principal types of chemical sensors and biosensors, definitions, measurement
principle.
2. Electrochemical sensors and microsensors, impedimetric sensors
3. Conductometric chemosensors, conductometric biosensors
4. Potentiometric sensors, amperometric sensors, pH sensors, polarographic sensors
5. Potentiometric biosensors and amperometric biosensors, bioelectrodes
6. Ion selective sensors, ISFET sensors, LAPS biosensors
7. Special electrochemical techniques - voltmetry, chronoamperometry, rotating techniques
8. Calorimetric biosensors, gravimetric chemo- and biosensors and microsensors
9. Temperature and optical chemo- and biosensors and microsensors
10. Fluorescence chemo- and biosensors and microsensors, chemiluminescence, bioluminescence
11. Special biosensosrs, enzyme biosensors, converters for bioaffinite sensors
12. Immunosensors, electrochemical biosensors for DNA
13. Physical sensors – temperature, flow, force, pressure, humidity, magnetic sensors, the Hall effect

Laboratory exercise

26 hours, compulsory

Teacher / Lecturer

Ing. Hana Kynclová, Ph.D.
Ing. Petra Majzlíková, Ph.D.
Ing. Jiří Sedláček
doc. Mgr. Markéta Vaculovičová, Ph.D.

Syllabus

1. Introduction to the measurement basics, measurement devices and laboratory tasks
2. Ultrasonic sensor
3. Detection of gases – O2 a CO2
4. Calorimetric sensor of the flow of fluids
5. Scanning of revolutions by means of the Hall sensor
6. Measurement of heavy metals by the potentiometric method
7. Measurement of pH solutions, conductivity measurement of water solutions
8. Amperometric biosensor
9. Humidity measurement
10. Pressure measurement
11. Lighting measurement
12. Temperature measurement
13. Substitute exercises