Czech

Not applicable.

Students receive during the course knowledge about basic unit operations of biotechnological process and their solution. Systematically will be stressed difference in solution with respect to special demands of biological . After passing this course students should be able to design simple bioengineering solution from cultivation side to isolation of products. Economic aspects of industrial production are to be considered. At this goal is also aiming mathematical modeling of biotechnological processes.

1 Structure and functions of microorganisms
2 Mass and energy balances, unit operations of chemical processes
3 Solution of ordinary differential equations, basic knowledge from analytical geometry, statistics and numerical mathematics.
4 Kinetics of chemical reactions and solution of simple kinetic equations

Not applicable.

Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

The scope of the course is determined by lectures/ seminars. Evaluation of the course is complex. In case of a small number of students it is possible to organize the course by means of distance learning. With few obligatory consultations.

Winter term

1 Flow characteristics in bioreactors. Mixing, aeration and oxygen transfer in bioreactors.
2 Industrial bioreactors. Construction of bioreactors, construction material. Auxiliary equipment. Media transport. Aseptic bioreactors.
3 Measurement, regulation and control of biotechnological processes.
4 Physiological state of micro-organisms, Cell transport in biological systems.
5 Kinetics of enzyme reactions.
6 Stoichiometry of microbial growth.
7 Kinetics of microbial growth and metabolite production.
8 Mathematical models of growth of microorganisms and product formation.
9 Survey on optimization methods.
10 Simulation language PSI.
11 Media, their preparation and sterilation. Air sterilation.
12 Fundamental cultivation techniques: batch, fed-batch, continuous and special techniques of cultivations (surface cultivation, cultivation on the interface between hydrophilic and hydrophobic phases, cultivation of plant and animal cells, production by means of immobilized cells).

Summer term
1 Separation techniques for product isolation and choice of separation method.
2 Membrane separations. Membranes. Microfiltration, ultrafiltration, nanofiltration, reverse osmosis.
3 Membrane separations. Membrane modules, arrangement of a membrane separation process. Electrodialysis. Pervaporation and permaeation of gases. Pervaporation. Membrane distillation. Dialysis.
4 Separations based on differences in densities.
5 Chromatographic methods and ion exchange.
6 Adsorption.
7 Extraction.
8 Evaporation.
9 Distillation,
10 Disintegration.
11 Methods for isolation of proteins (incl. enzymes).

Not applicable.

Aim of the course is to prepare students not only for application of bioengineering in from laboratory to large scale conditions but also to apply bioengineering methods and principles in solution of technological tasks and in solution of dissertation thesis. Extremely important is to understand sequence of operations of microbe, plant and animal cell cultivation and to balance each step of the process. Formulation of mathematical model of corresponding structure can help in optimization of cultivation conditions of cell cultivation and in isolation of microbial products.

Knowledge will be checked not only by oral exam but also elaborating and oral presentation of technological-engineering project.

Not applicable.

Not applicable.

M.Rychtera, J.Páca: Bioinženýrství kvasných procesů, 1985 (CS)
F.Kaštánek: Bioinženýrství, 2001 (CS)

M.Moo-Young:Comprehensive Biotechnology, Vol.2, 1985 (CS)
J.E.Baily, D.F.Ollis:Biochemical Engineering Fundamentals, 1986 (CS)
H.J.Rehm, G.Reed: Biiotechnology, Vol.3 (CS)
H.J.Rehm, G.Reed: Biotechnology, Vol.4 (CS)

0 hours, optionally