Course detail

Molecular Genetics

FIT-MOGAcad. year: 2010/2011

The course is an introduction into molecular biology and genetics. It describes the organization of genetic information  of procaryotic and eucaryotic genomes. It explains the principles of transcription and translation of genetic information and regulation of gene expression. Molecular mechanisms of mutagenesis and recombination, repair mechanisms of damaged DNA and mobile elements are also presented. The second part of the lecture is focused on the basic methods of molecular genetics and their applications in gene engineering in industry, agriculture and health care delivery.

Language of instruction

Czech

Number of ECTS credits

3

Mode of study

Not applicable.

Guarantor

prof. RNDr. Jiří Doškař, CSc.

Department

Faculty of Chemistry (FCH)

Learning outcomes of the course unit

Students will obtain the basic knowledge of genetic information,  expression of genes, its regulation and the methods allowing to modify genetic information.

Understanding the field which is not the main study component in a computer science-based study programme.

Prerequisites

The knowledge of biology and chemistry at the level of a high school study programme.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Study evaluation is based on marks obtained for specified items. Minimimum number of marks to pass is 50.

Course curriculum

  1. History of molecular genetics
  2. Genetic information and the genetic code
  3. Molecular structure of procaryotic and eucaryotic genome
  4. Replication and transcription of procaryotic and eucaryotic genome
  5. Postranscriptional modification and processing of RNA
  6. Translation and posttranslational processing
  7. Regulation of gene expression
  8. Molecular mechanisms of mutagenesis and recombination. Repair mechanisms of damaged DNA
  9. Mobile elements 
  10. Basic methods of molecular genetics 
  11. Basics of gene engineering
  12. Gene therapy. Genetic diseases and their diagnostics.
  13. Applications of molecular genetic

Work placements

Not applicable.

Aims

To understand the basic knowledge of genetic information, genes and genomes, expression of genes and its regulation. To understand the methods used in molecular genetics and gene engineering in research, industry, agriculture and medicine.

Specification of controlled education, way of implementation and compensation for absences

There are no checked study.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

  • Šmarda J. a kol.. Metody molekulární biologie, Brno, 2005.
  • Lewin B. Genes VII, Oxford University Press, Oxford, New York, Tokyo 2002.
  • Alberts et al.: Molecular biology of the cell. Garland Publ. 2004.
  • Clark D.: Molecular biology, Elsevier, 2005.
  • Watson J.D. et al., Recombinant DNA, 2nd ed., W.H. Freeman, New York 1992.

Recommended reading

  • Rosypal S. a kol.: Úvod do molekulární biologie. I.-IV. díl. Brno 1999-2002 (třetí vydání), 2006 - I. díl (čtvrté vydání)
  • Rosypal S. a kol.: Terminologie molekulární biologie, Brno 2001.
  • Alberts a kol: Základy buněčné biologie, Espero, 2000, 2005. 

Classification of course in study plans

  • Programme IT-MGR-2 Master's

    branch MIN , any year of study, winter semester, elective
    branch MBI , 1. year of study, winter semester, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

prof. RNDr. Jiří Doškař, CSc.

Syllabus

  1. History of molecular genetics
  2. Genetic information and the genetic code
  3. Molecular structure of procaryotic and eucaryotic genome
  4. Replication and transcription of procaryotic and eucaryotic genome
  5. Postranscriptional modification and processing of RNA
  6. Translation and posttranslational processing
  7. Regulation of gene expression
  8. Molecular mechanisms of mutagenesis and recombination. Repair mechanisms of damaged DNA
  9. Mobile elements 
  10. Basic methods of molecular genetics 
  11. Basics of gene engineering
  12. Gene therapy. Genetic diseases and their diagnostics.
  13. Applications of molecular genetic