Publication detail

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

ADAM,V. HÚSKA,D. KŘÍŽKOVÁ,S.HUBÁLEK,J.KIZEK,R.

Original Title

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

Type

journal article in Web of Science

Language

English

Original Abstract

Viral infections pose a threat for man kind. Diagnostic systems focus on the direct cultural proof of identity. Almost all systems use polymerase chain reaction. Real-time polymerase chain reaction is based on the polymerase chain reaction and routinely used to amplify and simultaneously quantify a targeted DNA molecule. Fluorescent dyes intercalating with double-stranded DNA and modified DNA oligonucleotide probes fluorescing when hybridized with a complementary DNA are used for real time quantification of the amplicons. The process of DNA quantification has demands on robust instrument and on high cost chemicals. Electrochemical detection is low cost and sensitive alternative method to detect nucleic acids. The main aim of this work is to propose novel electrochemical detector for monitoring PCR.

Keywords

Viral infections, double-stranded DNA, oligonucleotide probes, fluorescence, PCR

Authors

ADAM,V.; HÚSKA,D.; KŘÍŽKOVÁ,S.;HUBÁLEK,J.;KIZEK,R.

RIV year

2009

Released

4. 7. 2009

Publisher

FEBS JOURNAL

Location

Prague, CZECH REPUBLIC

ISBN

1742-464X

Periodical

FEBS Journal

Year of study

276

Number

Suppl. 1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

95

Pages to

96

Pages count

2

BibTex

@article{BUT93578,
  author="Vojtěch {Adam} and Dalibor {Húska} and Soňa {Křížková} and Jaromír {Hubálek} and René {Kizek}",
  title="Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses",
  journal="FEBS Journal",
  year="2009",
  volume="276",
  number="Suppl. 1",
  pages="95--96",
  issn="1742-464X"
}