Publication detail

DPPH aqueous antioxidant assay solution

SEDLÁŘ, K. SMERKOVÁ, K. ŠKUTKOVÁ, H. SOCHOR, J. ADAM, V. PROVAZNÍK, I. KIZEK, R.

Original Title

DPPH aqueous antioxidant assay solution

English Title

DPPH aqueous antioxidant assay solution

Type

conference paper

Language

en

Original Abstract

DPPH is a free radical commonly used in antioxidant assay. A purple coloured solution which becomes colourless after reaction with antioxidant is convenient to spectrofotometric analysis. Unfortunately, there are many protocols providing different results, so we are unable to compare them among laboratories. We tried to examine presumptions about stability of DPPH molecule as a common condition for many protocols. Using different temperatures and antioxidants (ascorbic acid, salicylic acid, homocysteine, etc.) we found out that some presumptions are wrong.

English abstract

DPPH is a free radical commonly used in antioxidant assay. A purple coloured solution which becomes colourless after reaction with antioxidant is convenient to spectrofotometric analysis. Unfortunately, there are many protocols providing different results, so we are unable to compare them among laboratories. We tried to examine presumptions about stability of DPPH molecule as a common condition for many protocols. Using different temperatures and antioxidants (ascorbic acid, salicylic acid, homocysteine, etc.) we found out that some presumptions are wrong.

Keywords

santioxidant assay, pectrofotometric analysis,

RIV year

2011

Released

01.06.2011

Location

Brno

ISBN

978-80-7375-514-0

Book

XI. Pracovní setkání fyzikálních chemiků a elektrochemiků

Pages from

250

Pages to

254

Pages count

5

BibTex


@inproceedings{BUT72564,
  author="Karel {Sedlář} and Kristýna {Šmerková} and Helena {Škutková} and Jiří {Sochor} and Vojtěch {Adam} and Ivo {Provazník} and René {Kizek}",
  title="DPPH aqueous antioxidant assay solution",
  annote="DPPH is a free radical commonly used in antioxidant assay. A purple coloured solution which becomes colourless after reaction with antioxidant is convenient to spectrofotometric analysis. Unfortunately, there are many protocols providing different results, so we are unable to compare them among laboratories. We tried to examine presumptions about stability of DPPH molecule as a common condition for many protocols. Using different temperatures and antioxidants (ascorbic acid, salicylic acid, homocysteine, etc.) we found out that some presumptions are wrong.",
  booktitle="XI. Pracovní setkání fyzikálních chemiků a elektrochemiků",
  chapter="72564",
  howpublished="print",
  year="2011",
  month="june",
  pages="250--254",
  type="conference paper"
}