Publication detail

Measurements of light scattering in an integrated microfluidic waveguide cytometer

SU, X. SINGH, K. CAPJACK, C. PETRÁČEK, J. BACKHOUSE, C.

Original Title

Measurements of light scattering in an integrated microfluidic waveguide cytometer

Type

journal article - other

Language

English

Original Abstract

An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

Keywords

light scattering, cytometry, cell analysis, waveguides, Mie theory

Authors

SU, X.; SINGH, K.; CAPJACK, C.; PETRÁČEK, J.; BACKHOUSE, C.

RIV year

2008

Released

1. 3. 2008

ISBN

1083-3668

Periodical

JOURNAL OF BIOMEDICAL OPTICS

Year of study

13

Number

2

State

United States of America

Pages from

024024-1

Pages to

024024-10

Pages count

10