Publication detail

Finite element simulation of mechanical tests of individual cells.

BURŠA, J. FUIS, V.

Original Title

Finite element simulation of mechanical tests of individual cells.

English Title

Finite element simulation of mechanical tests of individual cells.

Type

conference paper

Language

en

Original Abstract

The threedimensional finite element (FE) model of eucaryotic cell presented in the paper comprehends elements representing cell membrane, cytoplasm and nucleus, and a complex tensegrity structure representing cytoskeleton. In contrast to the previous models, this tensegrity structure consists of several parts. External and internal parts count 30 struts and 60 cables each and their corresponding nodes are interconnected by 30 radial members; these parts represent cortical, nuclear and deep cytoskeletons, respectively. This arrangement enables us to simulate the load transmission from the extracellular space via membrane receptors (focal adhesions) to the central part of the cell (nucleus, centrosome); this ability of the model was tested by simulation of some mechanical tests of isolated cells, in particular tension test with micropipettes, indentation test and magnetic tweezer test. Although material properties of compo-nents have been defined as realistic as possible on the base of the mechanical tests with vascular smooth muscle cells, they were not identified in fact and are not unique probably. However, simulations of the tests have shown the ability of the model to simulate the global load-deformation response of the cell under various types of loadings, as well as several substan-tial global features of the cell behaviour, e.g. "at a distance effect", non-linear stiffening with increasing load, or linear dependence of stiffness on increasing prestrain.

English abstract

The threedimensional finite element (FE) model of eucaryotic cell presented in the paper comprehends elements representing cell membrane, cytoplasm and nucleus, and a complex tensegrity structure representing cytoskeleton. In contrast to the previous models, this tensegrity structure consists of several parts. External and internal parts count 30 struts and 60 cables each and their corresponding nodes are interconnected by 30 radial members; these parts represent cortical, nuclear and deep cytoskeletons, respectively. This arrangement enables us to simulate the load transmission from the extracellular space via membrane receptors (focal adhesions) to the central part of the cell (nucleus, centrosome); this ability of the model was tested by simulation of some mechanical tests of isolated cells, in particular tension test with micropipettes, indentation test and magnetic tweezer test. Although material properties of compo-nents have been defined as realistic as possible on the base of the mechanical tests with vascular smooth muscle cells, they were not identified in fact and are not unique probably. However, simulations of the tests have shown the ability of the model to simulate the global load-deformation response of the cell under various types of loadings, as well as several substan-tial global features of the cell behaviour, e.g. "at a distance effect", non-linear stiffening with increasing load, or linear dependence of stiffness on increasing prestrain.

Keywords

Cell biomechanics, tensegrity structure, cytoskeleton, mechanical properties, finite element model

RIV year

2009

Released

07.09.2009

Publisher

Springer

Location

Munich, Germany

ISBN

978-3-642-03897-6

Book

IFMBE Proceedings

Pages from

16

Pages to

19

Pages count

4

Documents

BibTex


@inproceedings{BUT29749,
  author="Jiří {Burša} and Vladimír {Fuis}",
  title="Finite element simulation of mechanical tests of individual cells.",
  annote="The threedimensional finite element (FE) model of eucaryotic cell presented in the paper  comprehends elements representing cell membrane, cytoplasm and nucleus, and a complex tensegrity structure representing cytoskeleton. In contrast to the previous models, this tensegrity structure consists of several parts. External and internal parts count 30 struts and 60 cables each and their corresponding nodes are interconnected by 30 radial members; these parts represent cortical, nuclear and deep cytoskeletons, respectively. This arrangement enables us to simulate the load transmission from the extracellular space via membrane receptors (focal adhesions) to the central part of the cell (nucleus, centrosome); this ability of the model was tested by simulation of some mechanical tests of isolated cells, in particular tension test with micropipettes, indentation test and magnetic tweezer test. Although material properties of compo-nents have been defined as realistic as possible on the base of the mechanical tests with vascular smooth muscle cells, they were not identified in fact and are not unique probably. However, simulations of the tests have shown the ability of the model to simulate the global load-deformation response of the cell under various types of loadings, as well as several substan-tial global features of the cell behaviour, e.g. "at a distance effect", non-linear stiffening with increasing load, or linear dependence of stiffness on increasing prestrain.",
  address="Springer",
  booktitle="IFMBE Proceedings",
  chapter="29749",
  howpublished="electronic, physical medium",
  institution="Springer",
  year="2009",
  month="september",
  pages="16--19",
  publisher="Springer",
  type="conference paper"
}