Publication detail

Emission Properties Of Potential-Responsive Probe Di-4-ANEPPS

ČMIEL, V. MRAVEC, F. HALASOVÁ, T. SEKORA, J. PROVAZNÍK, I.

Original Title

Emission Properties Of Potential-Responsive Probe Di-4-ANEPPS

English Title

Emission Properties Of Potential-Responsive Probe Di-4-ANEPPS

Type

conference paper

Language

en

Original Abstract

Di-4-ANEPPS is a potential responsive probe. It is used to follow changes in membrane potential on perfused animal hearts. Di-4-ANEPPS and other probes from ANEPPS group respond to increases in membrane potential with a decrease in fluorescence excited at approximately 440 nm and an increase in fluorescence excited at 530 nm. This fluorescence shift and intensity change is used in fluorescence emission ratio measurements. Potential responsive probes are sensitive to solvent properties in these kind of measurements. We try to find out how much di-4-ANEPPS dependent on solvent polarity is.

English abstract

Di-4-ANEPPS is a potential responsive probe. It is used to follow changes in membrane potential on perfused animal hearts. Di-4-ANEPPS and other probes from ANEPPS group respond to increases in membrane potential with a decrease in fluorescence excited at approximately 440 nm and an increase in fluorescence excited at 530 nm. This fluorescence shift and intensity change is used in fluorescence emission ratio measurements. Potential responsive probes are sensitive to solvent properties in these kind of measurements. We try to find out how much di-4-ANEPPS dependent on solvent polarity is.

Keywords

Emission, Potential-Responsive Probe, Di-4-ANEPPS

RIV year

2010

Released

27.06.2010

Publisher

VUT v Brně

Location

Brno

ISBN

978-80-214-4106-4

Book

Analysis of Biomedical Signals and Images

Pages from

359

Pages to

363

Pages count

5

BibTex


@inproceedings{BUT25508,
  author="Vratislav {Čmiel} and Filip {Mravec} and Tereza {Venerová} and Jiří {Sekora} and Ivo {Provazník}",
  title="Emission Properties Of Potential-Responsive Probe Di-4-ANEPPS",
  annote="Di-4-ANEPPS is a potential responsive probe. It is used to follow changes in membrane potential on perfused animal hearts. Di-4-ANEPPS and other probes from ANEPPS group respond to increases in membrane potential with a decrease in fluorescence excited at approximately 440 nm and an increase in fluorescence excited at 530 nm. This fluorescence shift and intensity change is used in fluorescence emission ratio measurements. Potential responsive probes are sensitive to solvent properties in these kind of measurements. We try to find out how much di-4-ANEPPS dependent on solvent polarity is.",
  address="VUT v Brně",
  booktitle="Analysis of Biomedical Signals and Images",
  chapter="25508",
  howpublished="print",
  institution="VUT v Brně",
  year="2010",
  month="june",
  pages="359--363",
  publisher="VUT v Brně",
  type="conference paper"
}