Publication detail

Correlative Imaging of Malignant Melanomas Using Laser-Based Techniques, Histology, and Immunohistochemistry

KOPŘIVOVÁ, H. KISS, K. BRUNNBAUER, L. KRBAL, L. STEJSKAL, V. BUCHTOVÁ, M. KAŠKA, M. LIMBECK, A. POŘÍZKA, P. KAISER, J.

Original Title

Correlative Imaging of Malignant Melanomas Using Laser-Based Techniques, Histology, and Immunohistochemistry

Type

abstract

Language

English

Original Abstract

New methods are being sought to assist histologists in diagnosing tumors. Now, standard excision, the surgical removal of cancerous tissue, including a safety margin of healthy skin, followed by microscopic examination of the specimen, is most used for diagnosis. The problem is the correct estimation of the safety margin during tumor removal. Often incorrect tumor removal of insufficient safety margin leads to the need to repeat the surgery, which can be dangerous, especially for the elderly. Despite that histological examination is the leading diagnostic tool for skin cancer, there is an effort to discover a novel method suitable for preliminary screening. It has been shown that cancerous tissue changed the shape of the cells and their chemical composition, bioimaging techniques such as LIBS and LA-ICP-MS can be used to image the elemental composition of the tissue, which shows differences in composition of biotic (e.g., C, P, Ca, Mg) and trace (e.g., Zn, Cu) elements between healthy and cancerous tissue. A large sample set of human malignant melanomas was analyzed using LIBS and LA-ICP-MS, and spatial distributions of selected elements were constructed. Immunohistochemical studies were chosen to complete the biological information and confirm the tumor area. The antibody used was Melan A. The aim of this study is to show the potential of correlation of data obtained from all already mentioned methods, which could be used for the possible diagnosis of cancer as a complementary technique to classical histological examination. The next aim is to demonstrate on many malignant melanoma samples that the magnesium, which we can measure in a short time with and with a sufficient spatial resolution by LIBS, could serve as a biomarker for diagnosing skin cancer in the future

Keywords

Bioimaging, skin tumor, LIBS, LA-ICP-MS

Authors

KOPŘIVOVÁ, H.; KISS, K.; BRUNNBAUER, L.; KRBAL, L.; STEJSKAL, V.; BUCHTOVÁ, M.; KAŠKA, M.; LIMBECK, A.; POŘÍZKA, P.; KAISER, J.

Released

26. 10. 2023

BibTex

@misc{BUT186860,
  author="Hana {Kopřivová} and Kateřina {Kiss} and Lukas {Brunnbauer} and Lukáš {Krbal} and Václav {Stejskal} and Marcela {Buchtová} and Milan {Kaška} and Andreas {Limbeck} and Pavel {Pořízka} and Jozef {Kaiser}",
  title="Correlative Imaging of Malignant Melanomas Using Laser-Based Techniques, Histology, and Immunohistochemistry",
  year="2023",
  note="abstract"
}