Publication detail

Advanced correlative imaging of malignant melanomas using LIBS, LA-ICP-MS and immunohistochemistry

KOPŘIVOVÁ, H. KISS, K. BRUNNBAUER, L. KRBAL, L. STEJSKAL, V. BUCHTOVÁ, M. KAŠKA, M. LIMBECK, A. POŘÍZKA, P. KAISER, J.

Original Title

Advanced correlative imaging of malignant melanomas using LIBS, LA-ICP-MS and immunohistochemistry

Type

abstract

Language

English

Original Abstract

New methods are constantly being sought to assist histologists in diagnosing and analysing tumors. Now, standard excision, the surgical removal of cancerous tissue, including a safety margin of healthy skin, followed by microscopic examination of the specimen, is most used for diagnosis. The problem is the correct estimation of the safety margin during tumor removal. Often incorrect tumor removal of insufficient safety margin leads to the need to repeat the surgery, which can be dangerous, especially for the elderly. Despite that histological examination is the leading diagnostic tool for skin cancer, there is an effort to discover a novel method suitable for preliminary screening. Since cancerous tissue changed the shape of the cells and their chemical composition, bioimaging techniques such as LIBS and LA-ICP-MS can be used to image the elemental composition of the tissue, which shows differences in composition of biotic (e.g., C, P, Ca, Mg) and trace (e.g., Zn, Cu) elements between healthy and cancerous tissue. Analysis was performed using LIBS and LA-ICP-MS, and spatial distributions of selected elements were constructed. Immunohistochemical studies were chosen to complete the biological information and further confirm the tumor area. The antibody used was Melan A, which labels melanocytes and is used to diagnose malignant melanoma. The aim of this study is to show the potential of correlation of data obtained from all already mentioned methods. The next aim is to demonstrate on many malignant melanoma samples that the magnesium, which we can measure in a short time with and with a sufficient spatial resolution by LIBS, could serve as a biomarker for diagnosing skin cancer in the future.

Keywords

Bioimaging, skin tumor, laser ablation, soft tissues

Authors

KOPŘIVOVÁ, H.; KISS, K.; BRUNNBAUER, L.; KRBAL, L.; STEJSKAL, V.; BUCHTOVÁ, M.; KAŠKA, M.; LIMBECK, A.; POŘÍZKA, P.; KAISER, J.

Released

4. 9. 2023

BibTex

@misc{BUT186856,
  author="Hana {Kopřivová} and Kateřina {Kiss} and Lukas {Brunnbauer} and Lukáš {Krbal} and Václav {Stejskal} and Marcela {Buchtová} and Milan {Kaška} and Andreas {Limbeck} and Pavel {Pořízka} and Jozef {Kaiser}",
  title="Advanced correlative imaging of malignant melanomas using LIBS, LA-ICP-MS and immunohistochemistry",
  year="2023",
  note="abstract"
}