Publication detail

Gum Karaya based hydrogels for phage therapy of chronic wounds

ČERNÁ, E. VOJTOVÁ, L. BRTNÍKOVÁ, J. VACEK, L. RŮŽIČKA, F. FOHLEROVÁ, Z. PANTŮČEK, R. POSPÍŠIL, J. PLEVKA, P.

Original Title

Gum Karaya based hydrogels for phage therapy of chronic wounds

Type

abstract

Language

English

Original Abstract

The purpose of this work is to develop long-term stable hydrogel materials (freeze-dried films and injectable hydrogels) enriched with phages. Both developed materials proved to be biocompatible and to have sufficient transparency, swelling, and retention behavior and appropriate mechanical properties such as elasticity (up to 120 – 140 % from original size in the hydrated state), easy handling of the film, and optimal injectability (thixotropic character). Phage-enriched GK-hydrogels biostability was tested in three months using the double agar method, which proved phage activity and antibacterial properties against MRSA 1137. These tests proved the biostability of the injectable hydrogel after three months of experiments where the phage reached a concentration above 1·108 PFU/ml. Phage visualization in the hydrogel matrix was performed using fluorescent microscopy with labeled phages and GK to determine if they were present in the hydrogel matrix. Based on the results, we can conclude that GK-based hydrogels are a suitable carrier for phages even for long-term storage, treat S. aureus-infected chronic wounds, and support the healing process of infected wounds.

Keywords

gum Karaya, bacteriophage, antibacterial, stable, wound healing

Authors

ČERNÁ, E.; VOJTOVÁ, L.; BRTNÍKOVÁ, J.; VACEK, L.; RŮŽIČKA, F.; FOHLEROVÁ, Z.; PANTŮČEK, R.; POSPÍŠIL, J.; PLEVKA, P.

Released

26. 10. 2023

Location

Brno

Pages from

28

Pages to

28

Pages count

1

URL

https://drive.google.com/file/d/1TSTUdgBvfOhhfbUBjU5ceFjHqkLI3vyo/view?usp=sharing

BibTex

@misc{BUT185323,
  author="Eva {Černá} and Lucy {Vojtová} and Jana {Brtníková} and Lukáš {Vacek} and Filip {Růžička} and Zdenka {Fohlerová} and Roman {Pantůček} and Jakub {Pospíšil} and Pavel {Plevka}",
  title="Gum Karaya based hydrogels for phage therapy of chronic wounds",
  booktitle="Postdoc and PhD RETREAT Organised by CEITEC Postdoc and PhD committees, BOOK OF ABSTRACTS, 26.-27 October 2023, Milovy, Czechia",
  year="2023",
  pages="28--28",
  address="Brno",
  url="https://drive.google.com/file/d/1TSTUdgBvfOhhfbUBjU5ceFjHqkLI3vyo/view?usp=sharing",
  note="abstract"
}