Publication detail

Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development

Lopez, MG. Huteckova, B. Lavicky, J. Zezula, N. Rakultsev, V. Fridrichova, V. Tuaima, H. Nottmeier, C. Petersen, J. Kavkova, M. Zikmund, T. Kaiser, J. Lav, R. Star, H. Bryja, V. Henys, P. Vorechovsky, M. Tucker, AS. Harnos, J. Buchtova, M. Krivanek, J.

Original Title

Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development

Type

journal article in Web of Science

Language

English

Original Abstract

Mineralized tissues, such as bones or teeth, are essential structures of all vertebrates. They enable rapid movement, protection, and food processing, in addition to providing physiological functions. Although the development, regeneration, and pathogenesis of teeth and bones have been intensely studied, there is currently no tool to accurately follow the dynamics of growth and healing of these vital tissues in space and time. Here, we present the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach, which allows precise quantification of development, regeneration, remodeling, and healing in any type of calcified tissue across different species. Using mouse teeth as model the turnover rate of continuously growing incisors was quantified, and role of hard/soft diet on molar root growth was shown. Furthermore, the dynamics of bones and teeth growth in lizards, frogs, birds, and zebrafish was uncovered. This approach represents an effective, highly reproducible, and versatile tool that opens up diverse possibilities in developmental biology, bone and tooth healing, tissue engineering, and disease modeling.

Keywords

FLUOROCHROME LABELS; TETRACYCLINE; CALCEIN; SURFACE; XENOPUS; MODEL

Authors

Lopez, MG.; Huteckova, B.; Lavicky, J.; Zezula, N.; Rakultsev, V.; Fridrichova, V.; Tuaima, H.; Nottmeier, C.; Petersen, J.; Kavkova, M.; Zikmund, T.; Kaiser, J.; Lav, R.; Star, H.; Bryja, V.; Henys, P.; Vorechovsky, M.; Tucker, AS.; Harnos, J.; Buchtova, M. ; Krivanek, J.

Released

1. 8. 2023

Publisher

AMER ASSOC ADVANCEMENT SCIENCE

Location

WASHINGTON

ISBN

2375-2548

Periodical

Science Advances

Year of study

9

Number

31

State

United States of America

Pages count

17

URL

https://www.science.org/doi/10.1126/sciadv.adi0482

BibTex

@article{BUT184485,
  author="Lopez, MG. and Huteckova, B. and Lavicky, J. and Zezula, N. and Rakultsev, V. and Fridrichova, V. and Tuaima, H. and Nottmeier, C. and Petersen, J. and Kavkova, M. and Zikmund, T. and Kaiser, J. and Lav, R. and Star, H. and Bryja, V. and Henys, P. and Vorechovsky, M. and Tucker, AS. and Harnos, J. and Buchtova, M. and Krivanek, J.",
  title="Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development",
  journal="Science Advances",
  year="2023",
  volume="9",
  number="31",
  pages="17",
  doi="10.1126/sciadv.adi0482",
  issn="2375-2548",
  url="https://www.science.org/doi/10.1126/sciadv.adi0482"
}