Publication detail

White and black anodic TiO2 nanotubes: Comparison of biological effects in A549 and SH-SY5Y cells

SOPHA, H. BÁČOVÁ, J. BAISHYA, K. SEPÚLVEDA SEPÚLVEDA, L. RODRIGUEZ PEREIRA, J. ČAPEK, J. HROMÁDKO, L. ZAZPE MENDIOROZ, R. THALLURI, S. MISTRÍK, J. KNOTEK, P. ROUŠAR, T. MACÁK, J.

Original Title

White and black anodic TiO2 nanotubes: Comparison of biological effects in A549 and SH-SY5Y cells

Type

journal article in Web of Science

Language

English

Original Abstract

In this work, the cytotoxicity of black TiO2 nanotubes in comparison to their white counterparts is exploited for the first time. To provide a comprehensive overview, the toxicity is also assessed in comparison to multi-walled carbon nanotubes (MWCNTs) using two different cell lines. The TiO2 nanotube layers were prepared by anodic oxidation of Ti foils and investigated in the as-prepared white form, and in the black form, achieved by reduction via comproportionation reaction of Ti and TiO2. Additionally, a part of the black TiO2 nanotube layers was coated with a thin TiO2 coating with a nominal thickness of similar to 0.275 nm by Atomic Layer Deposition (ALD). Pulmonary A549 cells and neural SH-SY5Y cells were exposed to a wide range of TiO2 nanotube concentrations (1-500 mu g/mL) fo(r) 24 and 48 h. Then, the cytotoxicity was evaluated using cell viability and glutathione assessments. Essentially, no reduction in cell viability was observed after incubating the cells with TiO2 nanotubes up to 100 mu g/mL, while MWCNTs decreased the cell viability at a similar concentration significantly. In addition, ALD TiO2 coating on the black TiO2 nanotubes caused disappearance of mild toxic effects of white and black TiO2 nanotubes due to the shading of carbon and fluorine species incorporated within the TiO2 nanotube walls.

Keywords

TiO2 nanotube layers; Black TiO2; Cell growth; Cytotoxicity; Biocompatibility

Authors

SOPHA, H.; BÁČOVÁ, J.; BAISHYA, K.; SEPÚLVEDA SEPÚLVEDA, L.; RODRIGUEZ PEREIRA, J.; ČAPEK, J.; HROMÁDKO, L.; ZAZPE MENDIOROZ, R.; THALLURI, S.; MISTRÍK, J.; KNOTEK, P.; ROUŠAR, T.; MACÁK, J.

Released

15. 6. 2023

Publisher

Elsevier

Location

LAUSANNE

ISBN

1879-3347

Periodical

SURFACE & COATINGS TECHNOLOGY

Year of study

462

Number

1

State

Swiss Confederation

Pages from

1

Pages to

11

Pages count

11

URL

https://www.sciencedirect.com/science/article/pii/S0257897223002797

Full text in the Digital Library

http://hdl.handle.net/11012/213605

BibTex

@article{BUT184148,
  author="Hanna Ingrid {Sopha} and Jana {Báčová} and Kaushik {Baishya} and Lina Marcela {Sepúlveda Sepúlveda} and Jhonatan {Rodriguez Pereira} and Jan {Čapek} and Luděk {Hromádko} and Raúl {Zazpe Mendioroz} and Sitaramanjaneya Mouli {Thalluri} and Jan {Mistrík} and Petr {Knotek} and Tomáš {Roušar} and Jan {Macák}",
  title="White and black anodic TiO2 nanotubes: Comparison of biological effects in A549 and SH-SY5Y cells",
  journal="SURFACE & COATINGS TECHNOLOGY",
  year="2023",
  volume="462",
  number="1",
  pages="11",
  doi="10.1016/j.surfcoat.2023.129504",
  issn="1879-3347",
  url="https://www.sciencedirect.com/science/article/pii/S0257897223002797"
}