Publication detail

Antimicrobial Cost-Effective Transparent Hydrogel Films from Renewable Gum Karaya/Chitosan Polysaccharides for Modern Wound Dressings

DRÁPALOVÁ, E. MICHLOVSKÁ, L. POŠTULKOVÁ, H. CHAMRADOVÁ, I. LIPOVÝ, B. HOLOUBEK, J. VACEK, L. RŮŽIČKA, F. HANSLIANOVÁ, M. SVOBODOVÁ, T. ČERNÁ, E. HRDLIČKOVÁ, B. VOJTOVÁ, L.

Original Title

Antimicrobial Cost-Effective Transparent Hydrogel Films from Renewable Gum Karaya/Chitosan Polysaccharides for Modern Wound Dressings

Type

journal article in Web of Science

Language

English

Original Abstract

The newest trends in wound healing management and the development of the next generation of dressings are pointing toward natural polymeric materials with important beneficial properties such as antimicrobial effects, renewability, easier process of preparation, and biological activity. Here, we present the preparation and in vitro evaluation of a unique biopolymeric blend composed of natural polymers based on the positively charged polysaccharide chitosan and negatively charged gum karaya. A plate lysis assay of gum karaya and chitosan solution mixtures proved the synergistic antimicrobial effect against specific strains of both Gram-positive and Gram-negative bacteria and yeast. This polymeric mixture was used for hydrogel film preparation and determination of the composition effect on physical properties (swelling behavior in different solvents, pH, diffusion mechanism, hydrolytic stability, mechanical and optical properties). While the pure gum karaya with poly(vinyl alcohol) exhibited the highest hydrolytic degradation (68%), the mixture of poly(vinyl alcohol) and gum karaya with chitosan (in the 25:75 ratio) exhibited the lowest degradation value (41%) due to the strong physical interactions. Cytotoxicity tests performed with hydrogel extracts using two different in vitro models, adherent fibroblasts (NIH3T3) and non-adherent suspension B-lymphocytes (BaF3), exhibited excellent biocompatibility and no cytotoxicity. As expected, the antimicrobial activity of 3-day film extracts showed a significantly improved antimicrobial effect of mixtures involving a chitosan biopolymer. The physical and biological properties of prepared biopolymer-based hydrogels meet the requirements of modern wound dressings.

Keywords

natural gum; polysaccharides; biopolymers; antibacterial activity; cytotoxicity

Authors

DRÁPALOVÁ, E.; MICHLOVSKÁ, L.; POŠTULKOVÁ, H.; CHAMRADOVÁ, I.; LIPOVÝ, B.; HOLOUBEK, J.; VACEK, L.; RŮŽIČKA, F.; HANSLIANOVÁ, M.; SVOBODOVÁ, T.; ČERNÁ, E.; HRDLIČKOVÁ, B.; VOJTOVÁ, L.

Released

14. 4. 2023

Publisher

American Chemical Society

Location

WASHINGTON

ISBN

2637-6105

Periodical

ACS APPLIED POLYMER MATERIALS

Year of study

5

Number

4

State

United States of America

Pages from

2774

Pages to

2786

Pages count

13

URL

https://pubs.acs.org/doi/10.1021/acsapm.3c00025

Full text in the Digital Library

http://hdl.handle.net/11012/213684

BibTex

@article{BUT183757,
  author="Eva {Drápalová} and Lenka {Michlovská} and Hana {Poštulková} and Ivana {Chamradová} and Břetislav {Lipový} and Jakub {Holoubek} and Lukáš {Vacek} and Filip {Růžička} and Markéta {Hanslianová} and Táňa {Svobodová} and Eva {Černá} and Barbara {Hrdličková} and Lucy {Vojtová}",
  title="Antimicrobial Cost-Effective Transparent Hydrogel Films from Renewable Gum Karaya/Chitosan Polysaccharides for Modern Wound Dressings",
  journal="ACS APPLIED POLYMER MATERIALS",
  year="2023",
  volume="5",
  number="4",
  pages="2774--2786",
  doi="10.1021/acsapm.3c00025",
  issn="2637-6105",
  url="https://pubs.acs.org/doi/10.1021/acsapm.3c00025"
}