Publication detail

Imaging of Biological Tissues

POŘÍZKA, P. MODLITBOVÁ, P. KAISER, J.

Original Title

Imaging of Biological Tissues

Type

book chapter

Language

English

Original Abstract

Laser-Induced Breakdown Spectroscopy (LIBS) has established its position as a method for the analysis of biological samples. We aim to introduce the benefits of LIBS for the qualitative elemental imaging of tissues. The state-of-the-art instrumentation enables elemental imaging of sample surfaces with a high resolution (down to micro-scale) on a large scale (up to a whole-slide image), i.e., megapixel imaging of the distribution of investigated elements. The implementation of this promising tool to the analytical pipeline in various fields (from developmental biology to clinical research) is discussed. The typical interpretation of LIBS results through basic spectroscopic algorithms and machine learning is dissected. The chapter reviews recent advances of LIBS namely in soft and hard tissue analyses when the detection of endogenous and exogenous elements (major and trace metals) is concerned. The comparison to other elemental imaging methods (namely Laser Ablation Inductively Coupled Plasma Mass Spectrometry) is given. Finally, future perspectives and development of LIBS bioimaging is suggested.

Keywords

Laser-Induced Breakdown Spectroscopy (LIBS)

Authors

POŘÍZKA, P.; MODLITBOVÁ, P.; KAISER, J.

Released

13. 12. 2022

ISBN

978-3-031-14502-5

Book

Laser-Induced Breakdown Spectroscopy in Biological, Forensic and Materials Sciences

Pages count

26

URL

https://link.springer.com/chapter/10.1007/978-3-031-14502-5_6

BibTex

@inbook{BUT183026,
  author="Pavel {Pořízka} and Pavlína {Modlitbová} and Jozef {Kaiser}",
  title="Imaging of Biological Tissues",
  booktitle="Laser-Induced Breakdown Spectroscopy in Biological, Forensic and Materials Sciences",
  year="2022",
  pages="26",
  doi="10.1007/978-3-031-14502-5\{_}6",
  isbn="978-3-031-14502-5",
  url="https://link.springer.com/chapter/10.1007/978-3-031-14502-5_6"
}