Publication detail

Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration

KAUCKA, M. JOVEN ARAUS, A. TESAŘOVÁ, M. CURRIE, J. D. BOSTROM, J. KAVKOVÁ, M. PETERSEN, J. YAO, Z. BOUCHNITA, A. HELLANDER, A. ZIKMUND, T. ELEWA, A. NEWTON, P. T. FEI, J. CHAGIN, A. S. FRIED, K. TANAKA, E. M. KAISER, J. SIMON, A. ADAMEYKO, I.

Original Title

Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration

Type

journal article in Web of Science

Language

English

Original Abstract

There are major differences in duration and scale at which limb development and regeneration proceed, raising the question to what extent regeneration is a recapitulation of development. We address this by analyzing skeletal elements using a combination of micro-CT imaging, molecular profiling and clonal cell tracing. We find that, in contrast to development, regenerative skeletal growth is accomplished based entirely on cartilage expansion prior to ossification, not limiting the transversal cartilage expansion and resulting in bulkier skeletal parts. The oriented extension of salamander cartilage and bone appear similar to the development of basicranial synchondroses in mammals, as we found no evidence for cartilage stem cell niches or growth plate-like structures during neither development nor regeneration. Both regenerative and developmental ossification in salamanders start from the cortical bone and proceeds inwards, showing the diversity of schemes for the synchrony of cortical and endochondral ossification among vertebrates. Normal limb development relies on synchronized formation of cartilage and bone. Here, the authors show that in salamander limb regeneration these processes are decoupled: ossification occurs after the final size of regenerating cartilage is reached, allowing fast regeneration and leading to bulky bones.

Keywords

salamander; regeneration; cell division; microCT; molecular profiling; clonal cell tracing

Authors

KAUCKA, M.; JOVEN ARAUS, A.; TESAŘOVÁ, M.; CURRIE, J. D.; BOSTROM, J.; KAVKOVÁ, M.; PETERSEN, J.; YAO, Z.; BOUCHNITA, A.; HELLANDER, A.; ZIKMUND, T.; ELEWA, A.; NEWTON, P. T.; FEI, J.; CHAGIN, A. S.; FRIED, K.; TANAKA, E. M.; KAISER, J.; SIMON, A.; ADAMEYKO, I.

Released

14. 11. 2022

Publisher

NATURE PORTFOLIO

Location

BERLIN

ISBN

2041-1723

Periodical

NATURE COMMUNICATIONS

Year of study

13

Number

6949

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

17

Pages count

17

URL

https://www.nature.com/articles/s41467-022-34266-w

BibTex

@article{BUT180493,
  author="KAUCKA, M. and JOVEN ARAUS, A. and TESAŘOVÁ, M. and CURRIE, J. D. and BOSTROM, J. and KAVKOVÁ, M. and PETERSEN, J. and YAO, Z. and BOUCHNITA, A. and HELLANDER, A. and ZIKMUND, T. and ELEWA, A. and NEWTON, P. T. and FEI, J. and CHAGIN, A. S. and FRIED, K. and TANAKA, E. M. and KAISER, J. and SIMON, A. and ADAMEYKO, I.",
  title="Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration",
  journal="NATURE COMMUNICATIONS",
  year="2022",
  volume="13",
  number="6949",
  pages="1--17",
  doi="10.1038/s41467-022-34266-w",
  issn="2041-1723",
  url="https://www.nature.com/articles/s41467-022-34266-w"
}