Publication detail

SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

SMĚLÁ, D. CHANG, C.J. HROMÁDKO, L. MACÁK, J. BÍLKOVÁ, Z. TANIGUCHI, A.

Original Title

SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

Type

journal article in Web of Science

Language

English

Original Abstract

The immunoreactivity or/and stress response can be induced by nanomaterials' different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO2 nanofibers (SiO(2)NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO(2)NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-alpha and IL-1 beta in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO(2)NFs with a length approximate to 44 +/- 22 mu m that could induce ROS. Compared to this, only S-SiO(2)NFs with a length approximate to 14 +/- 17 mu m could enhance TNF-alpha and IL-1 beta expression. Our results suggested that L-SiO(2)NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO(2)NFs by THP-1 cells. The F-actin staining results indicated that SiO(2)NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO(2)NFs. However, our results suggested that the lengths of SiO(2)NFs induced different cellular responses.

Keywords

SiO2 nanofibers; cytotoxicity; immunoreactivity; THP-1-derived macrophage-like cells

Authors

SMĚLÁ, D.; CHANG, C.J.; HROMÁDKO, L.; MACÁK, J.; BÍLKOVÁ, Z.; TANIGUCHI, A.

Released

1. 7. 2022

Publisher

MDPI

Location

BASEL

ISBN

1420-3049

Periodical

MOLECULES

Year of study

27

Number

14

State

Swiss Confederation

Pages from

4456

Pages to

4468

Pages count

13

URL

https://www.mdpi.com/1420-3049/27/14/4456

BibTex

@article{BUT179258,
  author="SMĚLÁ, D. and CHANG, C.J. and HROMÁDKO, L. and MACÁK, J. and BÍLKOVÁ, Z. and TANIGUCHI, A.",
  title="SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells",
  journal="MOLECULES",
  year="2022",
  volume="27",
  number="14",
  pages="13",
  doi="10.3390/molecules27144456",
  issn="1420-3049",
  url="https://www.mdpi.com/1420-3049/27/14/4456"
}