Publication detail

Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure

HYMPANOVA, M. OLIVER-URRUTIA, C. VOJTA, M. MACHÁČEK, M. KŘUPKA, P. KUKLA, R. ČELKO, L. MONTUFAR, E. B. MAREK, J.

Original Title

Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure

Type

journal article in Web of Science

Language

English

Original Abstract

Streptococcus mutans is one of the bacteria that initiates the colonization of the pellicle at the tooth surface. It forms a plaque, together with other bacteria, which gradually dissolves the pellicle and leaves the tooth surface unprotected against the acidic oral environment. Calcium phosphate ceramics are excellent synthetic materials for the study of biofilm formation in dentistry because they are comparable to teeth in chemical composition and structure. Calcium phosphates can be processed to achieve a variety of crystalline compounds with biologically relevant ionic substitutions and structures that allow study of the effect of the surface chemistry and the topography independently. In this article, we describe the preparation and characterization of three types of calcium phosphate-based materials as a suitable surface for the formation of the S. mutans biofilm: beta-tricalcium phosphate (beta-TCP); sintered hydroxyapatite (SHA); and calcium-deficient hydroxyapatite (CDHA). The densest biofilms were formed on the surfaces of SHA and CDHA, with no significant differences due to the stoichiometry or microstructure. In contrast, beta-TCP showed a lower susceptibility to S. mutans biofilm formation, suggesting that the crystalline structure is the controlling parameter. Subsequently, SHA was selected to develop a dental biofilm model that allowed study of S. mutans biofilm susceptibility to chlorhexidine and ethanol.

Keywords

Calcium phosphate; Oral biofilm model; Streptococcus mutans; Biofilm; Bacteria

Authors

HYMPANOVA, M.; OLIVER-URRUTIA, C.; VOJTA, M.; MACHÁČEK, M.; KŘUPKA, P.; KUKLA, R.; ČELKO, L.; MONTUFAR, E. B.; MAREK, J.

Released

18. 3. 2022

Publisher

ELSEVIER

Location

AMSTERDAM, NETHERLANDS

ISBN

2772-9508

Periodical

BIOMATERIALS ADVANCES

Year of study

135

Number

212750

State

Kingdom of the Netherlands

Pages count

8

URL

https://www.sciencedirect.com/science/article/pii/S2772950822000279

BibTex

@article{BUT179190,
  author="HYMPANOVA, M. and OLIVER-URRUTIA, C. and VOJTA, M. and MACHÁČEK, M. and KŘUPKA, P. and KUKLA, R. and ČELKO, L. and MONTUFAR, E. B. and MAREK, J.",
  title="Assessment of Streptococcus mutans biofilm formation on calcium phosphate ceramics: The role of crystalline composition and microstructure",
  journal="BIOMATERIALS ADVANCES",
  year="2022",
  volume="135",
  number="212750",
  pages="8",
  doi="10.1016/j.bioadv.2022.212750",
  issn="2772-9508",
  url="https://www.sciencedirect.com/science/article/pii/S2772950822000279"
}