Publication detail

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

TURČANOVÁ, M. HRTOŇ, M. DVOŘÁK, P. NOVÁK, K. HERMANOVÁ, M. BEDNAŘÍK, Z. POLZER, S. BURŠA, J.

Original Title

Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

Type

journal article in Web of Science

Language

English

Original Abstract

A novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.

Keywords

soft tissue; histological slice; collagen; fiber direction; polarized light microscopy; histogram

Authors

TURČANOVÁ, M.; HRTOŇ, M.; DVOŘÁK, P.; NOVÁK, K.; HERMANOVÁ, M.; BEDNAŘÍK, Z.; POLZER, S.; BURŠA, J.

Released

28. 11. 2021

Publisher

Hindawi

Location

London, United Kingdom

ISBN

2314-6141

Periodical

BioMed Research International

Year of study

2021

Number

1

State

United States of America

Pages from

1

Pages to

13

Pages count

13

URL

https://www.hindawi.com/journals/bmri/2021/6879765/

Full text in the Digital Library

http://hdl.handle.net/11012/203098

BibTex

@article{BUT175037,
  author="Michaela {Turčanová} and Martin {Hrtoň} and Petr {Dvořák} and Kamil {Novák} and Markéta {Hermanová} and Zdeněk {Bednařík} and Stanislav {Polzer} and Jiří {Burša}",
  title="Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy",
  journal="BioMed Research International",
  year="2021",
  volume="2021",
  number="1",
  pages="1--13",
  doi="10.1155/2021/6879765",
  issn="2314-6141",
  url="https://www.hindawi.com/journals/bmri/2021/6879765/"
}