Publication detail

Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers

FOHLEROVÁ, Z. KAMNEV, K. SEPÚLVEDA SEPÚLVEDA, L. PYTLÍČEK, Z. PRÁŠEK, J. MOZALEV, A.

Original Title

Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers

Type

journal article in Web of Science

Language

English

Original Abstract

Here, zirconium-oxide ceramic coatings comprising arrays of 3D nanostructures are electrochemically synthesized, ranging in shape, size, spacing, and population density, termed as the nanomounds (o approximate to 65 nm), nanopillars (o approximate to 130 nm), and nanostumps (o approximate to 220 nm). The nanostructured coatings, alongside a flat ZrO2 anodic film, are explored as a potential biomaterial in experiments with Saos-2 cells. All coatings reveal no cytotoxicity to living cells. The population density and spreading area of the cells, being the largest on the flat film, slightly decrease with increasing nanostructure dimensions. The cells progressively proliferate on all the surfaces, the nanomounds and, especially, nanopillars promoting the best viabilities and proliferation rates. The flat, nanomound, and nanopillar coatings promote the well-defined organizations of actin filaments across the entire cell bodies with no disruption in the cytoskeletal network and the mature large dash-shaped focal adhesions. The highest activity of alkaline phosphatase and the biggest deposition of a mineralization-competent extracellular matrix occur on the nanopillar array, the other nanostructures showing a better result than the flat coating, though. The differences, paradoxes, and regularities in the cell responses are explained through the detailed consideration of the coating morphologies, surface chemistries, and processes at the cell/surface interfaces.

Keywords

anodizing; bio-interfaces; biomaterials; nanostructures; porous anodic alumina; Saos-2 cells; ZrO; (2)

Authors

FOHLEROVÁ, Z.; KAMNEV, K.; SEPÚLVEDA SEPÚLVEDA, L.; PYTLÍČEK, Z.; PRÁŠEK, J.; MOZALEV, A.

Released

25. 6. 2021

Publisher

WILEY

Location

HOBOKEN

ISBN

2196-7350

Periodical

Advanced Materials Interfaces

Number

10

State

Federal Republic of Germany

Pages from

1

Pages to

14

Pages count

14

URL

https://onlinelibrary.wiley.com/doi/10.1002/admi.202100256?af=R

BibTex

@article{BUT171982,
  author="Zdenka {Fohlerová} and Kirill {Kamnev} and Lina Marcela {Sepúlveda Sepúlveda} and Zdeněk {Pytlíček} and Jan {Prášek} and Alexander {Mozalev}",
  title="Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers",
  journal="Advanced Materials Interfaces",
  year="2021",
  number="10",
  pages="1--14",
  doi="10.1002/admi.202100256",
  issn="2196-7350",
  url="https://onlinelibrary.wiley.com/doi/10.1002/admi.202100256?af=R"
}