Publication detail

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

ČMIEL, V. SKOPALÍK, J. BAIAZITOVA, L. PROVAZNÍK, I.

Original Title

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

English Title

Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

Type

journal article

Language

en

Original Abstract

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral analysis in order to obtain an appropriate representation of the properties of the used dye in the original image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of its spectral properties.

English abstract

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral analysis in order to obtain an appropriate representation of the properties of the used dye in the original image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of its spectral properties.

Keywords

confocal microscopy; fluorescence; spectral scan; Stokes shift

Released

05.08.2019

Publisher

Cambridge University Press

Location

New York

Pages from

1264

Pages to

1265

Pages count

2

URL

BibTex


@article{BUT157958,
  author="Vratislav {Čmiel} and Josef {Skopalík} and Larisa {Baiazitova} and Ivo {Provazník}",
  title="Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy",
  annote="The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral
analysis in order to obtain an appropriate representation of the properties of the used dye in the original
image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat
cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of
its spectral properties.",
  address="Cambridge University Press",
  chapter="157958",
  doi="10.1017/S1431927619007050",
  howpublished="online",
  institution="Cambridge University Press",
  number="Suppl 2",
  volume="25",
  year="2019",
  month="august",
  pages="1264--1265",
  publisher="Cambridge University Press",
  type="journal article"
}