Publication detail

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

VANĚK, M. MRAVEC, F. SZOTKOWSKI, M. BYRTUSOVÁ, D. HÁRONIKOVÁ, A. MÁROVÁ, I.

Original Title

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

English Title

Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

Type

journal article in Web of Science

Language

en

Original Abstract

Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.

English abstract

Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.

Keywords

autofluorescence, carotenoids, Cystofilobasidium capitatum, fluorescence lifetime imaging, red yeasts

Released

10.04.2018

Publisher

De Gruyter Open

Location

Itálie

Pages from

114

Pages to

120

Pages count

7

URL

Documents

BibTex


@article{BUT155466,
  author="Martin {Vaněk} and Filip {Mravec} and Martin {Szotkowski} and Dana {Byrtusová} and Andrea {Němcová} and Ivana {Márová}",
  title="Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth",
  annote="Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determination of biomass and HPLC determination of pigments. To compare FLIM (Fluorescence Lifetime Imaging Microscopy) images and coupled data (obtained by CLSM) with model systems, FLIM analysis was performed on micelles of SDS:ergosterol and SDS:coenzyme Q with different content of ergosterol and coenzyme Q, respectively, and with constant addition of beta-carotene. Liposomes
lecithin:ergosterol:beta-carotene were investigated too. Two different intracellular forms of carotenoids were observed during most of cultivations, with third form appeared at the beginning of stationary phase. Observed behavior is probably due to formation of some kind of carotenoid protective system in compartments of yeast cell, especially cytoplasmic membrane.",
  address="De Gruyter Open",
  chapter="155466",
  doi="10.2478/ebtj-2018-0015",
  howpublished="online",
  institution="De Gruyter Open",
  number="2",
  volume="2",
  year="2018",
  month="april",
  pages="114--120",
  publisher="De Gruyter Open",
  type="journal article in Web of Science"
}