Publication detail

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

VOJTOVÁ, L. ZIKMUND, T. PAVLIŇÁKOVÁ, V. ŠALPLACHTA, J. KALASOVÁ, D. PROSECKÁ, E. BRTNÍKOVÁ, J. ŽÍDEK, J. PAVLIŇÁK, D. KAISER, J.

Original Title

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

English Title

The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography

Type

journal article in Web of Science

Language

en

Original Abstract

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

English abstract

This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.

Keywords

Biopolymeric scaffold, mesenchymal stem cells, SEM/EDX, tissue engineering, X-ray computed nanotomography

Released

01.03.2019

Publisher

WILEY

Location

HOBOKEN, USA

Pages from

169

Pages to

177

Pages count

9

URL

BibTex


@article{BUT151641,
  author="Lucy {Vojtová} and Tomáš {Zikmund} and Veronika {Pavliňáková} and Jakub {Šalplachta} and Dominika {Kalasová} and Eva {Prosecká} and Jana {Brtníková} and Jan {Žídek} and Jozef {Kaiser}",
  title="The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography",
  annote="This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume
of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation
of the contrasting agent – heavy metallic osmium tetroxide. The presented imaging technique is novel as it will
help to understand better the behaviour of cells while interacting with three-dimensional biomaterials. This is crucial for both experimental and clinical tissue engineering applications in order to limit the risk of uncontrolled cell growth, and potentially tumour formation.",
  address="WILEY",
  chapter="151641",
  doi="10.1111/jmi.12771",
  howpublished="print",
  institution="WILEY",
  number="3",
  volume="273",
  year="2019",
  month="march",
  pages="169--177",
  publisher="WILEY",
  type="journal article in Web of Science"
}