Publication detail

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

PEŠKOVÁ, M. HEGER, Z. DOSTÁLOVÁ, S. FOJTŮ, M. ČÁSTKOVÁ, K. ILKOVICS, L. VYKOUKAL, V. PEKAŘÍK, V.

Original Title

Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake

Type

journal article in Web of Science

Language

English

Original Abstract

Ceria nanoparticles can catalyze many redox reactions that resemble reactions catalyzed by naturally occurring enzymes, such as peroxidase, catalase, haloperoxidase or phosphatase. This makes nanoceria an attractive material for biomedical and sensing applications. Presence of detergents dramatically affects the catalytic activity of nanoceria. The catalytic activity is also strongly influenced by biogenic compounds present in the sample. Furthermore, biomedical applications are hindered by the difficulties to detect nanoceria uptake by cells. Therefore, we have developed a new fast and sensitive fluorescent haloperoxidase assay based on fluorescein bromination in presence of CTAB to detect uptake of CeO2 nanoparticles by cells in vitro. Moreover, catalytic activities of standard nanoceria (CeO2) were compared to nanoceria hydrates (CeO2.xH2O) that are easier for preparation and might be stabilized by biologically relevant molecules allowing for a specific targeting to cells or tissues. We have developed a new approach to produce CeO2.xH2O in presence of TEMED and analyzed whether the presence of H2O2 during the synthesis influences the catalytic activity of produced particles.

Keywords

Detergents · fluorescent detection · haloperoxidase, nanoceria hydrate · TEMED

Authors

PEŠKOVÁ, M.; HEGER, Z.; DOSTÁLOVÁ, S.; FOJTŮ, M.; ČÁSTKOVÁ, K.; ILKOVICS, L.; VYKOUKAL, V.; PEKAŘÍK, V.

Released

28. 9. 2018

ISBN

2365-6549

Periodical

ChemistrySelect

Year of study

3

Number

36

State

Federal Republic of Germany

Pages from

10139

Pages to

10146

Pages count

8

BibTex

@article{BUT151469,
  author="Marie {Pešková} and Zbyněk {Heger} and Simona {Dostálová} and Michaela {Fojtů} and Klára {Částková} and Ladislav {Ilkovics} and Vít {Vykoukal} and Vladimír {Pekařík}",
  title="Investigation of Detergent-Modified Enzymomimetic Activities of TEMED-Templated Nanoceria Towards Fluorescent Detection of Their Cellular Uptake",
  journal="ChemistrySelect",
  year="2018",
  volume="3",
  number="36",
  pages="10139--10146",
  doi="10.1002/slct.201802496",
  issn="2365-6549"
}