Publication detail

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

SVOBODA, O. BAIAZITOVA, L. ČMIEL, V. SKOPALÍK, J. PROVAZNÍK, I.

Original Title

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

English Title

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Type

conference paper

Language

en

Original Abstract

In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

English abstract

In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

Keywords

HL-1 cells, ASAP1, MEA.

Released

23.09.2018

Publisher

Computing in Cardiology

Location

Maastricht, Netherlands

Pages from

1

Pages to

4

Pages count

4

BibTex


@inproceedings{BUT150463,
  author="Ondřej {Svoboda} and Larisa {Baiazitova} and Vratislav {Čmiel} and Josef {Skopalík} and Ivo {Provazník}",
  title="Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation",
  annote="In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model.
HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses.
Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium 
(-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.",
  address="Computing in Cardiology",
  booktitle="Computing in Cardiology 2018",
  chapter="150463",
  doi="10.22489/CinC.2018.103",
  howpublished="online",
  institution="Computing in Cardiology",
  number="1",
  year="2018",
  month="september",
  pages="1--4",
  publisher="Computing in Cardiology",
  type="conference paper"
}